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PMID: 12080055 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sit4 is required for proper modulation of the biological functions mediated by Pkc1 and the cell integrity pathway in Saccharomyces cerevisiae.

The Journal of biological chemistry ·Vol. 277 ·No. 36 ·2002-09-06 ·Pages 33468-76

Angeles de la Torre-Ruiz M, Torres J, Arino J, Herrero E

Abstract

Maintenance of cellular integrity in Saccharomyces cerevisiae is carried out by the activation of the protein kinase C-mediated mitogen-activated protein kinase (PKC1-MAPK) pathway. Here we report that correct down-regulation of both basal and induced activity of the PKC1-MAPK pathway requires the SIT4 function. Sit4 is a protein phosphatase also required for a proper cell cycle progression. We present evidence demonstrating that the G(1) to S delay in the cell cycle, which occurs as a consequence of the absence of Sit4, is mediated by up-regulation of Pkc1 activity. Sit4 operates downstream of the plasma membrane sensors Mid2, Wsc1, and Wsc2 and upstream of Pkc1. Sit4 affects all known biological functions involving Pkc1, namely Mpk1 activity and cell wall integrity, actin cytoskeleton organization, and ribosomal gene transcription.

MeSH Terms
Acacia Actins/metabolism Calcium-Binding Proteins/metabolism Carrier Proteins/metabolism,physiology Cell Cycle Cell Wall/metabolism Cytoskeleton/metabolism Down-Regulation G1 Phase Immunoblotting Intracellular Signaling Peptides and Proteins Membrane Glycoproteins Membrane Proteins/metabolism Mitogen-Activated Protein Kinases Models, Biological Mutation Phosphoprotein Phosphatases Plant Proteins/metabolism Precipitin Tests Protein Binding Protein Kinase C/metabolism Protein Phosphatase 2 Protein Serine-Threonine Kinases/metabolism Ribosomes/metabolism S Phase Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins/metabolism Temperature Time Factors Transcription, Genetic Trypsin Inhibitors/metabolism Up-Regulation
Chemicals
Actins Calcium-Binding Proteins Carrier Proteins Intracellular Signaling Peptides and Proteins MID2 protein, S cerevisiae Membrane Glycoproteins Membrane Proteins Plant Proteins SLG1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Trypsin Inhibitors WSC2 protein, S cerevisiae ACTI protein, Acacia confusa Protein Serine-Threonine Kinases Protein Kinase C Mitogen-Activated Protein Kinases SLT2 protein, S cerevisiae Phosphoprotein Phosphatases Protein Phosphatase 2 SIT4 protein, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Angeles de la Torre-Ruiz Maria
Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina, Universitat de Lleida, Rovira Roure 44, 25198-Lleida, Spain. madellatorre@cmb.udl.es
Torres Jordi
Arino Joaquin
Herrero Enrique
Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
Published
2002-09-06
Epub
2002-00-21
Pages
33468-76
Language
English
Region
United States
NLM ID
2985121R
Subset
IM
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