Abstract
Maintenance of cellular integrity in Saccharomyces cerevisiae is carried out by the activation of the protein kinase C-mediated mitogen-activated protein kinase (PKC1-MAPK) pathway. Here we report that correct down-regulation of both basal and induced activity of the PKC1-MAPK pathway requires the SIT4 function. Sit4 is a protein phosphatase also required for a proper cell cycle progression. We present evidence demonstrating that the G(1) to S delay in the cell cycle, which occurs as a consequence of the absence of Sit4, is mediated by up-regulation of Pkc1 activity. Sit4 operates downstream of the plasma membrane sensors Mid2, Wsc1, and Wsc2 and upstream of Pkc1. Sit4 affects all known biological functions involving Pkc1, namely Mpk1 activity and cell wall integrity, actin cytoskeleton organization, and ribosomal gene transcription.
MeSH Terms
Acacia
Actins/metabolism
Calcium-Binding Proteins/metabolism
Carrier Proteins/metabolism,physiology
Cell Cycle
Cell Wall/metabolism
Cytoskeleton/metabolism
Down-Regulation
G1 Phase
Immunoblotting
Intracellular Signaling Peptides and Proteins
Membrane Glycoproteins
Membrane Proteins/metabolism
Mitogen-Activated Protein Kinases
Models, Biological
Mutation
Phosphoprotein Phosphatases
Plant Proteins/metabolism
Precipitin Tests
Protein Binding
Protein Kinase C/metabolism
Protein Phosphatase 2
Protein Serine-Threonine Kinases/metabolism
Ribosomes/metabolism
S Phase
Saccharomyces cerevisiae/metabolism
Saccharomyces cerevisiae Proteins/metabolism
Temperature
Time Factors
Transcription, Genetic
Trypsin Inhibitors/metabolism
Up-Regulation
Chemicals
Actins
Calcium-Binding Proteins
Carrier Proteins
Intracellular Signaling Peptides and Proteins
MID2 protein, S cerevisiae
Membrane Glycoproteins
Membrane Proteins
Plant Proteins
SLG1 protein, S cerevisiae
Saccharomyces cerevisiae Proteins
Trypsin Inhibitors
WSC2 protein, S cerevisiae
ACTI protein, Acacia confusa
Protein Serine-Threonine Kinases
Protein Kinase C
Mitogen-Activated Protein Kinases
SLT2 protein, S cerevisiae
Phosphoprotein Phosphatases
Protein Phosphatase 2
SIT4 protein, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Angeles de la Torre-Ruiz Maria
Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina, Universitat de Lleida, Rovira Roure 44, 25198-Lleida, Spain. madellatorre@cmb.udl.es
Torres Jordi
Arino Joaquin
Herrero Enrique