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PMID: 12077318 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The barley stem rust-resistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 14 ·2002-07-09 ·Pages 9328-33

Brueggeman R, Rostoks N, Kudrna D, Kilian A, Han F, Chen J, Druka A, Steffenson B, Kleinhofs A

Abstract

Stem rust caused by Puccinia graminis f. sp. tritici was among the most devastating diseases of barley in the northern Great Plains of the U.S. and Canada before the deployment of the stem rust-resistance gene Rpg1 in 1942. Since then, Rpg1 has provided durable protection against stem rust losses in widely grown barley cultivars (cvs.). Extensive efforts to clone Rpg1 by synteny with rice provided excellent flanking markers but failed to yield the gene because it does not seem to exist in rice. Here we report the map-based cloning and characterization of Rpg1. A high-resolution genetic map constructed with 8,518 gametes and a 330-kb bacterial artificial chromosome contig physical map positioned the gene between two crossovers approximately 0.21 centimorgan and 110 kb apart. The region including Rpg1 was searched for potential candidate genes by sequencing low-copy probes. Two receptor kinase-like genes were identified. The candidate gene alleles were sequenced from resistant and susceptible cvs. Only one of the candidate genes showed a pattern of apparently functional gene structure in the resistant cvs. and defective gene structure in the susceptible cvs. identifying it as the Rpg1 gene. Rpg1 encodes a receptor kinase-like protein with two tandem protein kinase domains, a novel structure for a plant disease-resistance gene. Thus, it may represent a new class of plant resistance genes.

MeSH Terms
Alleles Basidiomycota/pathogenicity Chromosome Mapping Chromosomes, Artificial, Bacterial/genetics DNA, Complementary/genetics DNA, Plant/genetics Edible Grain/genetics Genes, Plant Hordeum/enzymology,genetics,microbiology Molecular Sequence Data Oryza/genetics Physical Chromosome Mapping Plant Diseases/genetics,microbiology Receptor Protein-Tyrosine Kinases/genetics
Chemicals
DNA, Complementary DNA, Plant Receptor Protein-Tyrosine Kinases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Brueggeman R
Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6420, USA.
Rostoks N
Kudrna D
Kilian A
Han F
Chen J
Druka A
Steffenson B
Kleinhofs A
References (31)
31 references, click to expand
  1. Towards map-based cloning of the barley stem rust resistance genes Rpg1 and rpg4 using rice as an intergenomic cloning vehicle.
    Plant Mol Biol. 1997 Sep;35(1-2):187-95 PMID: 9291972
  2. Tomato Ve disease resistance genes encode cell surface-like receptors.
    Proc Natl Acad Sci U S A. 2001 May 22;98(11):6511-5 PMID: 11331751
  3. The Mla (powdery mildew) resistance cluster is associated with three NBS-LRR gene families and suppressed recombination within a 240-kb DNA interval on chromosome 5S (1HS) of barley.
    Genetics. 1999 Dec;153(4):1929-48 PMID: 10581297
  4. PLANT DISEASE RESISTANCE GENES.
    Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:575-607 PMID: 15012275
  5. Rapid reorganization of resistance gene homologues in cereal genomes.
    Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):370-5 PMID: 9419382
  6. Expression of a gibberellin-induced leucine-rich repeat receptor-like protein kinase in deepwater rice and its interaction with kinase-associated protein phosphatase.
    Plant Physiol. 1999 Jun;120(2):559-70 PMID: 10364408
  7. The evolution of disease resistance genes.
    Plant Mol Biol. 2000 Jan;42(1):195-204 PMID: 10688137
  8. Receptor kinase activation and signal transduction in plants: an emerging picture.
    Curr Opin Plant Biol. 2000 Oct;3(5):361-7 PMID: 11019802
  9. Genomic sequencing reveals gene content, genomic organization, and recombination relationships in barley.
    Funct Integr Genomics. 2002 May;2(1-2):51-9 PMID: 12021850
  10. Interaction of the maize and Arabidopsis kinase interaction domains with a subset of receptor-like protein kinases: implications for transmembrane signaling in plants.
    Plant J. 1997 Jul;12(1):83-95 PMID: 9263453
  11. Rice-barley synteny and its application to saturation mapping of the barley Rpg1 region.
    Nucleic Acids Res. 1995 Jul 25;23(14):2729-33 PMID: 7651834
  12. Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea.
    Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8816-20 PMID: 1681543
  13. Sequence analysis of a rice BAC covering the syntenous barley Rpg1 region
    Genome. 1999 Dec;42(6):1071-6 PMID: 10659772
  14. RFLP markers linked to the durable stem rust resistance gene Rpg1 in barley.
    Mol Plant Microbe Interact. 1994 Mar-Apr;7(2):298-301 PMID: 7912120
  15. The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
    Science. 1988 Jul 1;241(4861):42-52 PMID: 3291115
  16. Signal recognition and transduction mediated by the tomato Pto kinase: a paradigm of innate immunity in plants.
    Microbes Infect. 2000 Nov;2(13):1591-7 PMID: 11113378
  17. Map-based cloning of a protein kinase gene conferring disease resistance in tomato.
    Science. 1993 Nov 26;262(5138):1432-6 PMID: 7902614
  18. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  19. A barley gene family homologous to the maize rust resistance gene Rp1-D.
    Theor Appl Genet. 2002 Jun;104(8):1298-1306 PMID: 12582584
  20. A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome.
    Theor Appl Genet. 1993 Jul;86(6):705-12 PMID: 24193780
  21. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  22. Resistance gene complexes: evolution and utilization.
    Annu Rev Phytopathol. 2001;39:285-312 PMID: 11701867
  23. The S receptor kinase determines self-incompatibility in Brassica stigma.
    Nature. 2000 Feb 24;403(6772):913-6 PMID: 10706292
  24. Nested retrotransposons in the intergenic regions of the maize genome.
    Science. 1996 Nov 1;274(5288):765-8 PMID: 8864112
  25. A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21.
    Science. 1995 Dec 15;270(5243):1804-6 PMID: 8525370
  26. Evidence for two catalytically active kinase domains in pp90rsk.
    Mol Cell Biol. 1996 Mar;16(3):1212-9 PMID: 8622665
  27. SRK, the stigma-specific S locus receptor kinase of Brassica, is targeted to the plasma membrane in transgenic tobacco.
    Plant Cell. 1996 Mar;8(3):429-45 PMID: 8721749
  28. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
    Nucleic Acids Res. 1997 Sep 1;25(17):3389-402 PMID: 9254694
  29. Recognition specificity for the bacterial avirulence protein AvrPto is determined by Thr-204 in the activation loop of the tomato Pto kinase.
    Mol Cell. 1998 Aug;2(2):241-5 PMID: 9734361
  30. JAK3: a novel JAK kinase associated with terminal differentiation of hematopoietic cells.
    Oncogene. 1994 Aug;9(8):2415-23 PMID: 7518579
  31. Structure and function of the receptor-like protein kinases of higher plants.
    Plant Mol Biol. 1994 Dec;26(5):1599-609 PMID: 7858206
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.142284999
Published
2002-07-09
Epub
2002-00-20
Pages
9328-33
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC123140
Subset
IM
Databases
GENBANK
AF509744, AF509745, AF509746, AF509747, AF509748, AF509749, AF509750, AF509751, AF509752, AF509753, AF509754, AF509755, AF509756, AF509757, AF509758, AF509759, AF509760, AF509761, AF509762, AF509763, AF509764, AF509765, AF509766, AF509767, AF509768, AF509769, AF509770, AF509771, AF509772, AF509773, AF509774, AF509775, AF509776, AF509777, AF509778, AF509779
External Links
PubMed source DOI: 10.1073/pnas.142284999 PMC Full Text View journal details
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