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PMID: 12077248 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

NK cell receptors of the orangutan (Pongo pygmaeus): a pivotal species for tracking the coevolution of killer cell Ig-like receptors with MHC-C.

Journal of immunology (Baltimore, Md. : 1950) ·Vol. 169 ·No. 1 ·2002-07-01 ·Pages 220-9

Guethlein LA, Flodin LR, Adams EJ, Parham P

Abstract

CD94, NKG2, Ly49, and killer cell Ig-like receptor (KIR) expressed by orangutan peripheral blood cells were examined by cloning and sequencing cDNA from a panel of individuals. Orthologs of human CD94, NKG2A, D, and F were defined. NKG2C and E are represented by one gene, Popy-NKG2CE, that is equidistant from the two human genes. Several Popy-CD94, NKG2A, and NKG2CE alleles were defined. Popy-Ly49L is expressed in cultured NK cells and has a sequence consistent with it encoding a functional receptor. Orangutan KIR corresponding to the three KIR lineages expressed in humans and chimpanzees were defined. Popy-KIR2DL4 of lineage I is the only ortholog of a human or chimpanzee KIR, but in all individuals examined, the transcripts of this gene produced premature termination, either in the D2 domain or at the beginning of the cytoplasmic domain. Ten Popy-KIR3DL and one Popy-KIR3DS of lineage II are all closely related, but represent the products of at least two genes. The two Popy-KIR2DL and four Popy-KIR2DS of lineage III also represent two genes, both being more related to KIR2DS4 than to other human and chimpanzee KIR of lineage III. The Popy-KIR2D include ones predicted to be specific for the C1 epitope of MHC-C, but none specific for C2. This correlates with the observation that all orangutan MHC-C allotypes examined have the C1 motif.

MeSH Terms
Alleles Amino Acid Motifs/genetics,immunology Amino Acid Sequence Animals Antigens, CD/chemistry,genetics Antigens, Ly Evolution, Molecular HLA-C Antigens/chemistry,genetics Humans Killer Cells, Natural/immunology,metabolism Lectins/chemistry,genetics Lectins, C-Type Male Membrane Glycoproteins/chemistry,genetics,physiology Molecular Sequence Data NK Cell Lectin-Like Receptor Subfamily C NK Cell Lectin-Like Receptor Subfamily D Phylogeny Pongo pygmaeus/genetics,immunology Protein Binding/genetics,immunology Receptors, Immunologic/chemistry,genetics,metabolism Receptors, KIR Receptors, KIR2DL4 Receptors, Natural Killer Cell Sequence Homology, Amino Acid
Chemicals
Antigens, CD Antigens, Ly HLA-C Antigens KIR2DL4 protein, human KLRC1 protein, human KLRC2 protein, human KLRD1 protein, human Lectins Lectins, C-Type Ly-49L antigen Membrane Glycoproteins NK Cell Lectin-Like Receptor Subfamily C NK Cell Lectin-Like Receptor Subfamily D Receptors, Immunologic Receptors, KIR Receptors, KIR2DL4 Receptors, Natural Killer Cell
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Guethlein Lisbeth A
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305-5126, USA.
Flodin Laura R
Adams Erin J
Parham Peter
Article Info
Journal
Journal of immunology (Baltimore, Md. : 1950)
Abbr.
J Immunol
ISSN
0022-1767
DOI
10.4049/jimmunol.169.1.220
Published
2002-07-01
Pages
220-9
Language
English
Region
United States
NLM ID
2985117R
Subset
IM
Grants
NIAID NIH HHS · AI07290 · United States
NIAID NIH HHS · AI31168 · United States
Databases
GENBANK
AF470358, AF470359, AF470360, AF470361, AF470362, AF470363, AF470364, AF470365, AF470366, AF470367, AF470368, AF470369, AF470370, AF470371, AF470372, AF470373, AF470374, AF470375, AF470376, AF470377, AF470378, AF470379, AF470380, AF470381, AF470382, AF470383, AF470384, AF470385, AF470386, AF470387, AF470388, AF470389, AF470390, AF470391, AF470392, AF470393, AF470394, AF470395, AF470396, AF470397, AF470398, AF470399, AF470400, AF470401, AF470402, AF470403, AF470404, AF470405, AF470406
External Links
PubMed source DOI: 10.4049/jimmunol.169.1.220 View journal details
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