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PMID: 12052962 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Dynamics of thymocyte-stromal cell interactions visualized by two-photon microscopy.

Science (New York, N.Y.) ·Vol. 296 ·No. 5574 ·2002-06-07 ·Pages 1876-80

Bousso P, Bhakta NR, Lewis RS, Robey E

Abstract

Thymocytes are selected to mature according to their ability to interact with self major histocompatibility complex (MHC)-peptide complexes displayed on the thymic stroma. Using two-photon microscopy, we performed real-time analysis of the cellular contacts made by developing thymocytes undergoing positive selection in a three-dimensional thymic organ culture. A large fraction of thymocytes within these cultures were highly motile. MHC recognition was found to increase the duration of thymocyte-stromal cell interactions and occurred as both long-lived cellular associations displaying stable cell-cell contacts and as shorter, highly dynamic contacts. Our results identify the diversity and dynamics of thymocyte interactions during positive selection.

MeSH Terms

Animals Antigen Presentation Benzopyrans CD8-Positive T-Lymphocytes/immunology Cell Aggregation Cell Communication Cell Movement Cell Size Coculture Techniques Fluoresceins Fluorescent Dyes Histocompatibility Antigens Class I/immunology Lasers Mice Mice, Inbred C57BL Mice, Transgenic Microscopy/methods Naphthols Organ Culture Techniques Photons Receptors, Antigen, T-Cell/immunology Rhodamines Signal Transduction Stromal Cells/immunology,physiology Succinimides T-Lymphocyte Subsets/immunology T-Lymphocytes/cytology,immunology,physiology Thymus Gland/cytology,immunology Time Factors

Chemicals

5-(6)-carboxyfluorescein diacetate succinimidyl ester Benzopyrans Fluoresceins Fluorescent Dyes Histocompatibility Antigens Class I Naphthols Receptors, Antigen, T-Cell Rhodamines Succinimides seminaphthorhodaminefluoride

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Bousso Philippe

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.

Bhakta Nirav R

Lewis Richard S

Robey Ellen

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

1095-9203

DOI

10.1126/science.1070945

Published

2002-06-07

Pages

1876-80

Language

English

Region

United States

NLM ID

0404511

Subset

Grants

NIGMS NIH HHS · R01 GM045374 · United States

NIAID NIH HHS · AI32985 · United States

NIAID NIH HHS · AI42033 · United States

NIGMS NIH HHS · GM45374 · United States

Corrections

CommentIn

PMID: 12052942

External Links

PubMed source DOI: 10.1126/science.1070945 View journal details

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Dynamics of thymocyte-stromal cell interactions visualized by two-photon microscopy.

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