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PMID: 12034900 Published · ppublish English Journal Article

An Arabidopsis mitogen-activated protein kinase kinase kinase gene family encodes essential positive regulators of cytokinesis.

The Plant cell ·Vol. 14 ·No. 5 ·2002-05-00 ·Pages 1109-20

Krysan PJ, Jester PJ, Gottwald JR, Sussman MR

Abstract

The signal transduction pathways that control cytokinesis in plants are largely uncharacterized. Here, we provide genetic evidence that mitogen-activated protein kinase kinase kinases (MAPKKKs) play a role in the control of plant cell division. Using a reverse-genetic approach, we isolated plants carrying knockout alleles of the Arabidopsis MAPKKK genes ANP1, ANP2, and ANP3. The resulting single-mutant plants displayed no obvious abnormal phenotypes; two of the three double-mutant combinations displayed defects in cell division and growth; and the triple-mutant combination was not transmitted through either male or female gametes. The molecular and structural phenotypes displayed by the double mutants support a model in which the ANP family of MAPKKKs positively regulates cell division and growth and may negatively regulate stress responses.

MeSH Terms
Abscisic Acid/pharmacology Alleles Arabidopsis/enzymology,genetics,growth & development Brassinosteroids Cell Division/genetics Cholestanols/pharmacology Cytokinins/pharmacology Ethylenes/pharmacology Gene Expression Regulation, Plant/drug effects Genetic Complementation Test Gibberellins/pharmacology Hypocotyl/drug effects,growth & development,ultrastructure Indoleacetic Acids/pharmacology MAP Kinase Kinase Kinases/genetics,metabolism Microscopy, Electron Multigene Family Mutation Phenotype Plant Epidermis/drug effects,growth & development,ultrastructure Plant Growth Regulators/pharmacology Plant Stems/drug effects,growth & development,ultrastructure Plants, Genetically Modified Signal Transduction/genetics Steroids, Heterocyclic/pharmacology
Chemicals
Brassinosteroids Cholestanols Cytokinins Ethylenes Gibberellins Indoleacetic Acids Plant Growth Regulators Steroids, Heterocyclic Abscisic Acid ethylene ANP1 kinase MAP Kinase Kinase Kinases brassinolide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Krysan Patrick J
Biotechnology Center, University of Wisconsin-Madison, 425 Henry Mall, Madison, Wisconsin 53706, USA. fpat@biotech.wisc.edu
Jester Peter J
Gottwald Jennifer R
Sussman Michael R
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.001164
Email
fpat@biotech.wisc.edu
Published
2002-05-00
Pages
1109-20
Language
English
Region
England
NLM ID
9208688
PMCID
PMC150610
Subset
IM
Corrections
CommentIn
PMID: 12034889
External Links
PubMed source DOI: 10.1105/tpc.001164 PMC Full Text View journal details
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