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PMID: 12034837 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Evidence that DNase I hypersensitive site 5 of the human beta-globin locus control region functions as a chromosomal insulator in transgenic mice.

Nucleic acids research ·Vol. 30 ·No. 11 ·2002-06-01 ·Pages 2484-91

Li Q, Zhang M, Han H, Rohde A, Stamatoyannopoulos G

Abstract

We have previously reported that DNase I hypersensitive site 5 (5'HS5) of the human beta-globin locus control region functions as a chromatin insulator in stable transfection assays. In this report we show that a 3.2 kb DNA fragment containing the entire 5'HS5 region can protect a position-sensitive (A)gamma-globin gene against position effects in transgenic mice. Bracketing is required for function of 5'HS5 as an insulator. The 5'HS5 insulator operates in adult as well as in embryonic murine erythroid cells. The insulator has no significant stimulatory effects of its own. These results indicate that 5'HS5 can function as a chromatin insulator in vivo.

MeSH Terms
Aging/blood,genetics Animals Chromatin/chemistry,genetics,metabolism Cytomegalovirus/genetics DNA, Recombinant/genetics Deoxyribonuclease I/metabolism Embryonic and Fetal Development Enhancer Elements, Genetic/genetics Female Gene Expression Regulation, Developmental Globins/genetics Humans Integrases/genetics Locus Control Region/genetics Male Mice Mice, Transgenic RNA, Messenger/genetics,metabolism Transgenes/genetics Viral Proteins/genetics Yolk Sac/metabolism
Chemicals
Chromatin DNA, Recombinant RNA, Messenger Viral Proteins Globins Cre recombinase Integrases Deoxyribonuclease I
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Li Qiliang
Division of Medical Genetics, Box 357720, Department of Medicine, University of Washington Medical School, Seattle, WA 98195, USA.
Zhang Miaohua
Han Hemei
Rohde Alex
Stamatoyannopoulos George
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/30.11.2484
Published
2002-06-01
Pages
2484-91
Language
English
Region
England
NLM ID
0411011
PMCID
PMC117184
Subset
IM
Grants
NIDDK NIH HHS · R56 DK045365 · United States
NIDDK NIH HHS · R37 DK045365 · United States
NHLBI NIH HHS · P01 HL053750 · United States
NHLBI NIH HHS · HL53750 · United States
NIDDK NIH HHS · R01 DK045365 · United States
NIDDK NIH HHS · DK45365 · United States
External Links
PubMed source DOI: 10.1093/nar/30.11.2484 PMC Full Text View journal details
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