Home LiteratureArticle Details
PMID: 12034830 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interactions between p53, hMSH2-hMSH6 and HMG I(Y) on Holliday junctions and bulged bases.

Nucleic acids research ·Vol. 30 ·No. 11 ·2002-06-01 ·Pages 2427-34

Subramanian D, Griffith JD

Abstract

The ability of the tumor suppressor protein, p53, to recognize certain types of DNA lesions may represent one of the mechanisms by which this protein modulates cellular response to DNA damage. p53 DNA binding properties are regulated by several factors, such as post-translational modifications including phosphorylation and acetylation, regulation by its own C-terminal domain and interactions with other cellular proteins. Substrates resembling Holliday junctions and extra base bulges were used to study the effect of three nuclear proteins, HMG-1, HMG I(Y) and hMSH2-hMSH6, on the lesion binding properties of p53. Gel retardation assays revealed that the three proteins had varying effects on p53 binding to these substrates. HMG-1 did not influence p53 binding to Holliday junctions or 3-cytosine bulges. HMG I(Y) rapidly dissociated p53 complexes with Holliday junctions but not 3-cytosine bulges. Finally, the mismatch repair protein complex, hMSH2-hMSH6, enhanced p53 binding to both substrates by 3-4-fold. Together, these results demonstrate that p53 DNA binding activity is highly influenced by the presence of other proteins, some having a dominant effect while others have a negative effect.

MeSH Terms
Animals Base Pair Mismatch Cattle Cytosine/chemistry,metabolism DNA/chemistry,genetics,metabolism DNA Repair DNA-Binding Proteins/metabolism HMGA1a Protein/metabolism Humans MutS Homolog 2 Protein Nucleic Acid Conformation Protein Binding Proto-Oncogene Proteins/metabolism Recombination, Genetic Tumor Suppressor Protein p53/metabolism
Chemicals
DNA-Binding Proteins G-T mismatch-binding protein Proto-Oncogene Proteins Tumor Suppressor Protein p53 HMGA1a Protein Cytosine DNA MSH2 protein, human MutS Homolog 2 Protein
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Subramanian Deepa
Lineberger Comprehensive Cancer Center and Department of Microbiology and Immunology, CB 7295 Mason Farm Road, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295, USA.
Griffith Jack D
References (50)
50 references, click to expand
  1. p53 binds single-stranded DNA ends through the C-terminal domain and internal DNA segments via the middle domain.
    Nucleic Acids Res. 1995 Feb 11;23(3):362-9 PMID: 7885831
  2. Changes in superhelicity are introduced into closed circular DNA by binding of high mobility group protein I/Y.
    J Biol Chem. 1995 Mar 3;270(9):4355-60 PMID: 7876198
  3. Activation of p53 sequence-specific DNA binding by short single strands of DNA requires the p53 C-terminus.
    Cell. 1995 Jun 30;81(7):1021-9 PMID: 7600571
  4. p53 is linked directly to homologous recombination processes via RAD51/RecA protein interaction.
    EMBO J. 1996 Apr 15;15(8):1992-2002 PMID: 8617246
  5. p53: puzzle and paradigm.
    Genes Dev. 1996 May 1;10(9):1054-72 PMID: 8654922
  6. The XPB and XPD DNA helicases are components of the p53-mediated apoptosis pathway.
    Genes Dev. 1996 May 15;10(10):1219-32 PMID: 8675009
  7. High-mobility-group chromosomal proteins: architectural components that facilitate chromatin function.
    Prog Nucleic Acid Res Mol Biol. 1996;54:35-100 PMID: 8768072
  8. Saccharomyces cerevisiae MSH2, a mispaired base recognition protein, also recognizes Holliday junctions in DNA.
    J Mol Biol. 1997 Jan 24;265(3):289-301 PMID: 9018043
  9. p53, the cellular gatekeeper for growth and division.
    Cell. 1997 Feb 7;88(3):323-31 PMID: 9039259
  10. Human p53 binds Holliday junctions strongly and facilitates their cleavage.
    J Biol Chem. 1997 Mar 14;272(11):7532-9 PMID: 9054458
  11. Identification of redox/repair protein Ref-1 as a potent activator of p53.
    Genes Dev. 1997 Mar 1;11(5):558-70 PMID: 9119221
  12. Competition between HMG-I(Y), HMG-1 and histone H1 on four-way junction DNA.
    Nucleic Acids Res. 1997 Sep 1;25(17):3523-31 PMID: 9254714
  13. The human mismatch recognition complex hMSH2-hMSH6 functions as a novel molecular switch.
    Cell. 1997 Dec 26;91(7):995-1005 PMID: 9428522
  14. HMG box proteins bind to four-way DNA junctions in their open conformation.
    EMBO J. 1998 Feb 2;17(3):817-26 PMID: 9451006
  15. High mobility group protein-1 (HMG-1) is a unique activator of p53.
    Genes Dev. 1998 Feb 15;12(4):462-72 PMID: 9472015
  16. How p53 binds DNA as a tetramer.
    EMBO J. 1998 Jun 15;17(12):3342-50 PMID: 9628871
  17. Specific mismatch recognition in heteroduplex intermediates by p53 suggests a role in fidelity control of homologous recombination.
    Mol Cell Biol. 1998 Sep;18(9):5332-42 PMID: 9710617
  18. DNA damage activates p53 through a phosphorylation-acetylation cascade.
    Genes Dev. 1998 Sep 15;12(18):2831-41 PMID: 9744860
  19. Surface plasmon resonance measurements reveal stable complex formation between p53 and DNA polymerase alpha.
    Oncogene. 1999 Jan 21;18(3):769-74 PMID: 9989827
  20. Introduction: p53--the first twenty years.
    Cell Mol Life Sci. 1999 Jan;55(1):9-11 PMID: 10065147
  21. Covalent and noncovalent modifiers of the p53 protein.
    Cell Mol Life Sci. 1999 Jan;55(1):76-87 PMID: 10065153
  22. 'Saccharomyces cerevisiae MSH2/6 complex interacts with Holliday junctions and facilitates their cleavage by phage resolution enzymes.
    J Biol Chem. 1999 Mar 12;274(11):7200-6 PMID: 10066781
  23. p53-mediated apoptosis is attenuated in Werner syndrome cells.
    Genes Dev. 1999 Jun 1;13(11):1355-60 PMID: 10364153
  24. The role of mismatched nucleotides in activating the hMSH2-hMSH6 molecular switch.
    J Biol Chem. 2000 Feb 11;275(6):3922-30 PMID: 10660545
  25. HMG I/Y regulates long-range enhancer-dependent transcription on DNA and chromatin by changes in DNA topology.
    Nucleic Acids Res. 2000 Jul 1;28(13):2541-50 PMID: 10871404
  26. Analysis of the binding of p53 to DNAs containing mismatched and bulged bases.
    J Biol Chem. 2001 Mar 23;276(12):8778-84 PMID: 11124254
  27. Interaction with p53 enhances binding of cisplatin-modified DNA by high mobility group 1 protein.
    J Biol Chem. 2001 Mar 9;276(10):7534-40 PMID: 11106654
  28. p53 protein at the hub of cellular DNA damage response pathways through sequence-specific and non-sequence-specific DNA binding.
    Carcinogenesis. 2001 Jun;22(6):851-60 PMID: 11375889
  29. Chromatin immunoprecipitation analysis fails to support the latency model for regulation of p53 DNA binding activity in vivo.
    Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):95-100 PMID: 11756653
  30. Specific recognition of cruciform DNA by nuclear protein HMG1.
    Science. 1989 Feb 24;243(4894 Pt 1):1056-9 PMID: 2922595
  31. Spontaneous and UV-induced mutations in Escherichia coli K-12 strains with altered or absent DNA polymerase I.
    J Bacteriol. 1989 May;171(5):2480-4 PMID: 2651403
  32. The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants.
    Nature. 1989 Nov 23;342(6248):396-401 PMID: 2555716
  33. Cleavage specificity of bacteriophage T4 endonuclease VII and bacteriophage T7 endonuclease I on synthetic branch migratable Holliday junctions.
    J Mol Biol. 1990 Apr 20;212(4):723-35 PMID: 2158565
  34. Efficient large-scale purification of non-histone chromosomal proteins HMG1 and HMG2 by using Polybuffer-exchanger PBE94.
    J Chromatogr. 1990 Aug 24;530(1):39-46 PMID: 2277118
  35. Participation of p53 protein in the cellular response to DNA damage.
    Cancer Res. 1991 Dec 1;51(23 Pt 1):6304-11 PMID: 1933891
  36. Phosphorylation by cdc2 kinase modulates DNA binding activity of high mobility group I nonhistone chromatin protein.
    J Biol Chem. 1991 Oct 25;266(30):19945-52 PMID: 1939057
  37. Regulation of the specific DNA binding function of p53.
    Cell. 1992 Nov 27;71(5):875-86 PMID: 1423635
  38. Wild-type p53 adopts a 'mutant'-like conformation when bound to DNA.
    EMBO J. 1993 Mar;12(3):1021-8 PMID: 8458320
  39. Structure of the HMG box motif in the B-domain of HMG1.
    EMBO J. 1993 Apr;12(4):1311-9 PMID: 8467791
  40. The acidic transcriptional activation domains of VP16 and p53 bind the cellular replication protein A and stimulate in vitro BPV-1 DNA replication.
    Cell. 1993 Jun 18;73(6):1207-21 PMID: 8390328
  41. Solution structure of a DNA-binding domain from HMG1.
    Nucleic Acids Res. 1993 Jul 25;21(15):3427-36 PMID: 8346022
  42. Interaction of high mobility group-I (Y) nonhistone proteins with nucleosome core particles.
    J Biol Chem. 1993 Oct 5;268(28):21137-46 PMID: 8407950
  43. DNA strand breaks: the DNA template alterations that trigger p53-dependent DNA damage response pathways.
    Mol Cell Biol. 1994 Mar;14(3):1815-23 PMID: 8114714
  44. The DNA-bending protein HMG-1 enhances progesterone receptor binding to its target DNA sequences.
    Mol Cell Biol. 1994 May;14(5):3376-91 PMID: 8164686
  45. Increased UV-induced SCEs but normal repair of DNA damage in p53-deficient mouse cells.
    Int J Cancer. 1994 Jul 15;58(2):254-7 PMID: 8026887
  46. Purified human MSH2 protein binds to DNA containing mismatched nucleotides.
    Cancer Res. 1994 Nov 1;54(21):5539-42 PMID: 7923193
  47. Alternatively spliced forms in the carboxy-terminal domain of the p53 protein regulate its ability to promote annealing of complementary single strands of nucleic acids.
    Mol Cell Biol. 1995 Jan;15(1):497-504 PMID: 7528329
  48. Insect proteins homologous to mammalian high mobility group proteins I/Y (HMG I/Y). Characterization and binding to linear and four-way junction DNA.
    J Biol Chem. 1994 Dec 30;269(52):33042-8 PMID: 7806532
  49. Interaction between cisplatin-modified DNA and the HMG boxes of HMG 1: DNase I footprinting and circular dichroism.
    J Mol Biol. 1995 Feb 17;246(2):243-7 PMID: 7869375
  50. p53 and its 14 kDa C-terminal domain recognize primary DNA damage in the form of insertion/deletion mismatches.
    Cell. 1995 Jun 30;81(7):1013-20 PMID: 7600570
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/30.11.2427
Published
2002-06-01
Pages
2427-34
Language
English
Region
England
NLM ID
0411011
PMCID
PMC117204
Subset
IM
Grants
NIGMS NIH HHS · R01 GM031819 · United States
NCI NIH HHS · CA70343 · United States
NIGMS NIH HHS · GM31819 · United States
External Links
PubMed source DOI: 10.1093/nar/30.11.2427 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2