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PMID: 12029062 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Requirement for NusG for transcription antitermination in vivo by the lambda N protein.

Journal of bacteriology ·Vol. 184 ·No. 12 ·2002-06-00 ·Pages 3416-8

Zhou Y, Filter JJ, Court DL, Gottesman ME, Friedman DI

Abstract

Transcription antitermination by the bacteriophage lambda N protein is stimulated in vitro by the Escherichia coli NusG protein. Earlier work suggested that NusG was not required for N activity in vivo. Here we present evidence that NusG also stimulates N-mediated transcription antitermination in intact cells.

MeSH Terms
Bacterial Proteins/metabolism Bacteriophage lambda/metabolism Escherichia coli/virology Escherichia coli Proteins Peptide Elongation Factors/metabolism Terminator Regions, Genetic Transcription Factors/metabolism Transcription, Genetic Viral Regulatory and Accessory Proteins/metabolism
Chemicals
Bacterial Proteins Escherichia coli Proteins N protein, Bacteriophage lambda NusG protein, E coli Peptide Elongation Factors Transcription Factors Viral Regulatory and Accessory Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zhou Ying
Department of Microbiology and Immunology, The University of Michigan, Medical School, Ann Arbor, Michigan 48109-0620, USA.
Filter Joshua J
Court Donald L
Gottesman Max E
Friedman David I
References (25)
25 references, click to expand
  1. Regulation of rho-dependent transcription termination by NusG is specific to the Escherichia coli elongation complex.
    Biochemistry. 2000 May 9;39(18):5573-85 PMID: 10820031
  2. Processive antitermination.
    J Bacteriol. 1999 Jan;181(2):359-67 PMID: 9882646
  3. Bacteriophage lambda: alive and well and still doing its thing.
    Curr Opin Microbiol. 2001 Apr;4(2):201-7 PMID: 11282477
  4. Studies of novel transducing variants of lambda: dispensability of genes N and Q.
    Virology. 1969 Oct;39(2):348-52 PMID: 5344297
  5. Prevention of the lethality of induced lambda prophage by an isogenic lambda plasmid.
    Virology. 1972 Nov;50(2):472-81 PMID: 4564526
  6. Interference with the expression of the N gene function of phage lambda in a mutant of Escherichia coli.
    Virology. 1973 Jan;51(1):216-26 PMID: 4567743
  7. Genetic characterization of a bacterial locus involved in the activity of the N function of phage lambda.
    Virology. 1974 Mar;58(1):141-8 PMID: 4595374
  8. Host interference with expression of the lambda N gene product.
    Biochimie. 1974;56(11-12):1505-9 PMID: 4619340
  9. Cooperative effects of bacterial mutations affecting lambda N gene expression. I. Isolation and characterization of a nusB mutant.
    Virology. 1976 Aug;73(1):119-27 PMID: 785802
  10. Evidence that ribosomal protein S10 participates in control of transcription termination.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):1115-8 PMID: 6453343
  11. Escherichia coli nusB mutations that suppress nusA1 exhibit lambda N specificity.
    J Mol Biol. 1983 Jul 25;168(1):73-85 PMID: 6224023
  12. Sequence and transcriptional pattern of the essential Escherichia coli secE-nusG operon.
    J Bacteriol. 1990 Mar;172(3):1621-7 PMID: 2137819
  13. Analysis of the Escherichia coli nusA10(Cs) allele: relating nucleotide changes to phenotypes.
    J Bacteriol. 1991 Feb;173(4):1485-91 PMID: 1847364
  14. Assembly of transcription elongation complexes containing the N protein of phage lambda and the Escherichia coli elongation factors NusA, NusB, NusG, and S10.
    Genes Dev. 1991 Aug;5(8):1504-12 PMID: 1831176
  15. Effect of Escherichia coli nusG function on lambda N-mediated transcription antitermination.
    J Bacteriol. 1992 Feb;174(4):1339-44 PMID: 1531224
  16. Requirement for E. coli NusG protein in factor-dependent transcription termination.
    Cell. 1992 Mar 6;68(5):989-94 PMID: 1547498
  17. NusG, a new Escherichia coli elongation factor involved in transcriptional antitermination by the N protein of phage lambda.
    J Biol Chem. 1992 Mar 25;267(9):6012-9 PMID: 1532577
  18. How the phage lambda N gene product suppresses transcription termination: communication of RNA polymerase with regulatory proteins mediated by signals in nascent RNA.
    J Bacteriol. 1992 Nov;174(21):6711-6 PMID: 1400223
  19. Elongation factor NusG interacts with termination factor rho to regulate termination and antitermination of transcription.
    Genes Dev. 1993 Jan;7(1):161-72 PMID: 8422985
  20. A quaternary transcription termination complex. Reciprocal stabilization by Rho factor and NusG protein.
    J Mol Biol. 1994 Nov 11;243(5):830-9 PMID: 7525972
  21. NusG is required to overcome a kinetic limitation to Rho function at an intragenic terminator.
    Proc Natl Acad Sci U S A. 1995 May 23;92(11):4738-42 PMID: 7761393
  22. Transcription termination signals in the nin region of bacteriophage lambda: identification of Rho-dependent termination regions.
    Genetics. 1995 Jul;140(3):875-87 PMID: 7672588
  23. NusG overexpression inhibits Rho-dependent termination in Escherichia coli.
    Mol Microbiol. 1995 Aug;17(4):633-41 PMID: 8801418
  24. Transcription antitermination: the lambda paradigm updated.
    Mol Microbiol. 1995 Oct;18(2):191-200 PMID: 8709839
  25. An efficient recombination system for chromosome engineering in Escherichia coli.
    Proc Natl Acad Sci U S A. 2000 May 23;97(11):5978-83 PMID: 10811905
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.184.12.3416-3418.2002
Published
2002-06-00
Pages
3416-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC135089
Subset
IM
Grants
NIGMS NIH HHS · R01 GM037219 · United States
NIGMS NIH HHS · R56 GM037219 · United States
PHS HHS · A111459-10 · United States
NIGMS NIH HHS · GM37219 · United States
External Links
PubMed source DOI: 10.1128/JB.184.12.3416-3418.2002 PMC Full Text View journal details
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