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PMID: 1202021 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Organization of an actin filament-membrane complex. Filament polarity and membrane attachment in the microvilli of intestinal epithelial cells.

The Journal of cell biology ·Vol. 67 ·No. 3 ·1975-12-00 ·Pages 725-43

Mooseker MS, Tilney LG

Abstract

The association of actin filaments with membranes is now recognized as an important parameter in the motility of nonmuscle cells. We have investigated the organization of one of the most extensive and highly ordered actin filament-membrane complexes in nature, the brush border of intestinal epithelial cells. Through the analysis of isolated, demembranated brush borders decorated with the myosin subfragment, S1, we have determined that all the microvillar actin filaments have the same polarity. The S1 arrowhead complexes point away from the site of attachment of actin filaments at the apical tip of the microvillar membrane. In addition to the end-on attachment of actin filaments at the tip of the microvillus, these filaments are also connected to the plasma membrane all along their lengths by periodic (33 nm) cross bridges. These bridges were best observed in isolated brush borders incubated in high concentrations of Mg++. Their visibility is attributed to the induction of actin paracrystals in the filament bundles of the microvilli. Finally, we present evidence for the presence of myosinlike filaments in the terminal web region of the brush border. A model for the functional organization of actin and myosin in the brush border is presented.

MeSH Terms
Actins/analysis Animals Cell Membrane/ultrastructure Chickens Epithelial Cells Epithelium/analysis,drug effects,ultrastructure Intestinal Mucosa/analysis,drug effects,ultrastructure Magnesium/pharmacology Models, Biological Myosins/analysis Polyethylene Glycols/pharmacology
Chemicals
Actins Polyethylene Glycols Myosins Magnesium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mooseker M S
Tilney L G
References (39)
39 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.67.3.725
Published
1975-12-00
Pages
725-43
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2111646
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.67.3.725 PMC Full Text View journal details
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