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PMID: 12019132 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning of a cation efflux pump gene associated with chlorhexidine resistance in Klebsiella pneumoniae.

Antimicrobial agents and chemotherapy ·Vol. 46 ·No. 6 ·2002-06-00 ·Pages 2024-8

Fang CT, Chen HC, Chuang YP, Chang SC, Wang JT

Abstract

Expression libraries of a chlorhexidine-resistant Klebsiella pneumoniae strain were constructed and transformed into Escherichia coli XLOLR. Twenty chlorhexidine-resistant transformants were obtained after selection. All clones contained a novel 903-nucleotide locus. Its sequences were compatible with a cation efflux pump, and the locus was thus designated as cepA. Retransformation using cepA-containing plasmids conferred chlorhexidine resistance to both XLOLR and a chlorhexidine-sensitive K. pneumoniae strain. Therefore, CepA is associated with chlorhexidine resistance and may act as a cation efflux pump.

MeSH Terms
Amino Acid Sequence Anti-Infective Agents, Local/pharmacology Base Sequence Cation Transport Proteins/genetics,metabolism Chlorhexidine/pharmacology DNA, Bacterial/genetics Drug Resistance, Microbial Escherichia coli/genetics Gene Library Klebsiella pneumoniae/drug effects,genetics Molecular Sequence Data Open Reading Frames Plasmids/genetics
Chemicals
Anti-Infective Agents, Local Cation Transport Proteins DNA, Bacterial Chlorhexidine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fang Chi-Tai
Department of Internal Medicine, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.
Chen Haur-Chuan
Chuang Yi-Ping
Chang Shan-Chwen
Wang Jin-Town
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.46.6.2024-2028.2002
Published
2002-06-00
Pages
2024-8
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC127239
Subset
IM
Databases
GENBANK
AB073019
External Links
PubMed source DOI: 10.1128/AAC.46.6.2024-2028.2002 PMC Full Text View journal details
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