Establishment of two cell lines from embryonic tissue of the tobacco hornworm, Manduca sexta (L).

In vitro ·Vol. 11 ·No. 6 ·1975-00-00 ·Pages 395-9

Eide PE, Caldwell JM, Marks EP

Abstract

Two cell lines were derived from primary cultures of embryonic tissue of the tobacco hornworm, Manduca sexta (L.). The cell lines were maintained on hemolymph-free synthetic insect medium. Cytogenetic and immunoligical identification of the lines were carried out. Techniques for obtaining the line and medium, subculturieng and freezing procedures for long-term storage, and the morphological and growth characteristics are described.

MeSH Terms

Cell Aggregation Cell Division Cell Line Cell Nucleus/ultrastructure Cell Survival Cells, Cultured/ultrastructure Culture Media Cytoplasmic Granules/ultrastructure Freezing Karyotyping Lepidoptera/cytology Vacuoles/ultrastructure

Chemicals

Culture Media

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Eide P E

Caldwell J M

Marks E P

References (9)

9 references, click to expand

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Cellular Response to Ecdysterone in vitro.
Science. 1969 Sep 26;165(3900):1374-5 PMID: 17817888
Chromosome preparations of leukocytes cultured from human peripheral blood.
Exp Cell Res. 1960 Sep;20:613-6 PMID: 13772379
Establishment of a cell line from long-term primary embryonic house fly cell cultures.
J Insect Physiol. 1975 Aug;21(8):1431-8 PMID: 1159306
Adaptation of an insect cell line (Grace's Antheraea cells) to medium free of insect hemolymph.
Science. 1967 Mar 24;155(3769):1565-6 PMID: 6020481
Effects of ecdysterone in vitro on hindgut and hemocytes of Manduca sexta (Lepidopteral).
Gen Comp Endocrinol. 1971 Oct;17(2):351-9 PMID: 4937769
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Proc Natl Acad Sci U S A. 1973 May;70(5):1509-13 PMID: 16592086

Article Info

Journal

In vitro

Abbr.

In Vitro

ISSN

0073-5655

DOI

10.1007/BF02616376

Published

1975-00-00

Pages

395-9

Language

English

Region

United States

NLM ID

0063733

Subset

External Links

PubMed source DOI: 10.1007/BF02616376

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Establishment of two cell lines from embryonic tissue of the tobacco hornworm, Manduca sexta (L).

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