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PMID: 12011028 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of the spv locus in Salmonella enterica serovar Arizona.

Infection and immunity ·Vol. 70 ·No. 6 ·2002-06-00 ·Pages 3290-4

Libby SJ, Lesnick M, Hasegawa P, Kurth M, Belcher C, Fierer J, Guiney DG

Abstract

Salmonella enterica serovar Arizona (S. enterica subspecies IIIa) is a common Salmonella isolate from reptiles and can cause serious systemic disease in humans. The spv virulence locus, found on large plasmids in Salmonella subspecies I serovars associated with severe infections, was confirmed to be located on the chromosome of serovar Arizona. Sequence analysis revealed that the serovar Arizona spv locus contains homologues of spvRABC but lacks the spvD gene and contains a frameshift in spvA, resulting in a different C terminus. The SpvR protein functions as a transcriptional activator for the spvA promoter, and SpvB and SpvC are highly conserved. The analysis supports the proposal that the chromosomal spv sequence more closely corresponds to the ancestral locus acquired during evolution of S. enterica, with plasmid acquisition of spv genes in the subspecies I strains involving addition of spvD and polymorphisms in spvA.

MeSH Terms
ADP Ribose Transferases/genetics Amino Acid Sequence Animals Antigens, Bacterial Bacterial Proteins/genetics Carrier Proteins/genetics Conserved Sequence Frameshift Mutation Gene Deletion Humans Molecular Sequence Data Promoter Regions, Genetic Reptiles/microbiology Salmonella Infections/microbiology Salmonella arizonae/genetics,isolation & purification Sequence Homology, Amino Acid Virulence Factors
Chemicals
Antigens, Bacterial Bacterial Proteins Carrier Proteins SpvD protein, Salmonella enterica Virulence Factors tccA protein, IncP plasmid RK2 ADP Ribose Transferases spvB protein, Salmonella enterica virulence plasmid
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Libby Stephen J
Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27695-7615, USA.
Lesnick Marc
Hasegawa Patricia
Kurth Michael
Belcher Christopher
Fierer Joshua
Guiney Donald G
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.70.6.3290-3294.2002
Published
2002-06-00
Pages
3290-4
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC127997
Subset
IM
Grants
NIDDK NIH HHS · P01 DK035108 · United States
NIDDK NIH HHS · DK35108 · United States
NIAID NIH HHS · AI-32178 · United States
NIAID NIH HHS · R01 AI032178 · United States
NIAID NIH HHS · R56 AI032178 · United States
External Links
PubMed source DOI: 10.1128/IAI.70.6.3290-3294.2002 PMC Full Text View journal details
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