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PMID: 11988092 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Gastric MUC5AC and MUC6 are large oligomeric mucins that differ in size, glycosylation and tissue distribution.

The Biochemical journal ·Vol. 364 ·No. Pt 1 ·2002-05-15 ·Pages 191-200

Nordman H, Davies JR, Lindell G, de Bolós C, Real F, Carlstedt I

Abstract

Gastric MUC5AC and MUC6 mucins were studied using polyclonal antibodies. Immunohistochemistry showed MUC5AC to originate from the surface epithelium, whereas MUC6 was produced by the glands. Mucins from the surface epithelium or glands of corpus and antrum were purified using CsCl/4M guanidinium chloride density-gradient centrifugation. MUC5AC appeared as two distinct populations at 1.4 and 1.3 g/ml, whereas MUC6, which was enriched in the gland tissue, appeared at 1.45 g/ml. Reactivity with antibodies against the Le(b) structure (where Le represents the Lewis antigen) followed the MUC5AC distribution, whereas antibodies against the Le(y) structure and reactivity with the GlcNAc-selective Solanum tuberosum lectin coincided with MUC6, suggesting that the two mucins are glycosylated differently. Rate-zonal centrifugation of whole mucins and reduced subunits showed that both gastric MUC5AC and MUC6 are oligomeric glycoproteins composed of disulphide-bond linked subunits and that oligomeric MUC5AC was apparently smaller than MUC6. A heterogeneous population of 'low-density' MUC5AC mucins, which were smaller than the 'high-density' ones both before and after reduction, reacted with an antibody against a variable number tandem repeat sequence within MUC5AC, suggesting that they represent precursor forms of this mucin. Following ion-exchange HPLC, both MUC5AC and MUC6 appeared as several distinct populations, probably corresponding to 'glycoforms' of the mucins, the most highly charged of which were found in the gland tissue.

MeSH Terms
Centrifugation, Density Gradient Chromatography Chromatography, High Pressure Liquid Enzyme-Linked Immunosorbent Assay Epithelial Cells Gastric Mucosa/metabolism Glycosylation Humans Immunohistochemistry Mucin 5AC Mucin-6 Mucins/biosynthesis,chemistry Protein Isoforms Protein Structure, Tertiary Tissue Distribution
Chemicals
MUC5AC protein, human MUC6 protein, human Mucin 5AC Mucin-6 Mucins Protein Isoforms
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Nordman Henrik
Mucosal Biology Group, Department of Cell and Molecular Biology, Level C13, BMC Lund University, S-221 84, Lund, Sweden.
Davies Julia R
Lindell Gert
de Bolós Carme
Real Francisco
Carlstedt Ingemar
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj3640191
Published
2002-05-15
Pages
191-200
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1222561
Subset
IM
External Links
PubMed source DOI: 10.1042/bj3640191 PMC Full Text View journal details
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