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PMID: 11983900 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The LEF1/beta -catenin complex activates movo1, a mouse homolog of Drosophila ovo required for epidermal appendage differentiation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 9 ·2002-04-30 ·Pages 6064-9

Li B, Mackay DR, Dai Q, Li TW, Nair M, Fallahi M, Schonbaum CP, Fantes J, Mahowald AP, Waterman ML, Fuchs E, Dai X

Abstract

Drosophila ovo/svb (dovo) is required for epidermal cuticle/denticle differentiation and is genetically downstream of the wg signaling pathway. Similarly, a mouse homolog of dovo, movo1, is required for the proper formation of hair, a mammalian epidermal appendage. Here, we provide biochemical evidence that movo1 encodes a nuclear DNA binding protein (mOvo1a) that binds to DNA sequences similar to those that dOvo binds to, further supporting the notion that mOvo1a and dOvo are genetically and biochemically homologous proteins. Additionally, we show that the movo1 promoter is activated by the lymphoid enhancer factor 1 (LEF1)/beta-catenin complex, a transducer of wnt signaling. Collectively, our findings suggest that movo1 is a developmental target of wnt signaling during hair morphogenesis in mice, and that the wg/wnt-ovo link in epidermal appendage regulatory pathways has been conserved between mice and flies.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Cell Differentiation Cell Nucleus/metabolism Chromosomes/metabolism Cloning, Molecular Cytoskeletal Proteins/metabolism DNA, Complementary/metabolism DNA-Binding Proteins/metabolism,physiology Deoxyribonuclease I/metabolism Epidermal Cells Epidermis/embryology Immunoblotting Immunohistochemistry Lymphoid Enhancer-Binding Factor 1 Mice Microscopy, Fluorescence Molecular Sequence Data Promoter Regions, Genetic Protein Binding Recombinant Proteins/metabolism Sequence Homology, Amino Acid Trans-Activators Transcription Factors/metabolism,physiology Transfection beta Catenin
Chemicals
CTNNB1 protein, mouse Cytoskeletal Proteins DNA, Complementary DNA-Binding Proteins Lef1 protein, mouse Lymphoid Enhancer-Binding Factor 1 Ovo1 protein, mouse Recombinant Proteins Trans-Activators Transcription Factors beta Catenin Deoxyribonuclease I
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Li Baoan
Department of Biological Chemistry, University of California, Irvine, CA 92697, USA.
Mackay Douglas R
Dai Qian
Li Tony W H
Nair Mahalakshmi
Fallahi Magid
Schonbaum Christopher P
Fantes Judith
Mahowald Anthony P
Waterman Marian L
Fuchs Elaine
Dai Xing
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.092137099
Published
2002-04-30
Pages
6064-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC122902
Subset
IM
Grants
NIAMS NIH HHS · R01 AR047320 · United States
NIAMS NIH HHS · R01 AR47320 · United States
Databases
GENBANK
AF487891
External Links
PubMed source DOI: 10.1073/pnas.092137099 PMC Full Text View journal details
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