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PMID: 11983257 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Muscle specific versus ubiquitous expression of Gag based HIV-1 DNA vaccines: a comparative analysis.

Vaccine ·Vol. 20 ·No. 15 ·2002-05-06 ·Pages 1975-9

Bojak A, Hammer D, Wolf H, Wagner R

Abstract

Most studies on DNA-based immunization have used viral promoters to drive antigen expression. In this study, we analyzed the properties of the commonly used CMV promoter, the tissue specific murine muscle creatine kinase (MCK) promoter as well as a hybrid MCK/CMV promoter regarding promoter activity and tissue specificity in vitro. Furthermore, the efficiency of inducing HIV-1 Gag specific immune responses in vivo following intramuscular immunization of naked DNA containing a codon optimized synthetic gene was compared. Although antibody titers and cellular immune responses using the MCK construct were slightly reduced as compared to conventional CMV based vector modules, the utilization of nonviral promoters may add significantly to the safety of future DNA vaccines.

MeSH Terms

AIDS Vaccines/genetics,immunology Animals Antibody Specificity Creatine Kinase/genetics Creatine Kinase, MM Form Cytomegalovirus/genetics Enhancer Elements, Genetic Female Gene Products, gag/genetics,immunology Genes, Reporter Genes, Synthetic Genes, gag HIV Antibodies/biosynthesis,immunology HIV Core Protein p24/genetics,immunology HIV-1/genetics,immunology Humans Isoenzymes/genetics Luciferases/biosynthesis,genetics Lung Neoplasms/pathology Mice Mice, Inbred BALB C Muscle, Skeletal/metabolism Organ Specificity Promoter Regions, Genetic Protein Precursors/genetics,immunology Transcription, Genetic Tumor Cells, Cultured Vaccines, DNA/genetics,immunology Vaccines, Synthetic/genetics,immunology

Chemicals

AIDS Vaccines Gene Products, gag HIV Antibodies HIV Core Protein p24 Isoenzymes Protein Precursors Vaccines, DNA Vaccines, Synthetic p55 gag precursor protein, Human immunodeficiency virus 1 Luciferases Creatine Kinase Creatine Kinase, MM Form

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Bojak Alexandra

Institute of Medical Microbiology, University of Regensburg, Franz-Josef-Strauss Allee 11, 93053 Regensburg, Germany.

Hammer Diana

Wolf Hans

Wagner Ralf

Article Info

Journal

Vaccine

Abbr.

Vaccine

ISSN

0264-410X

DOI

10.1016/s0264-410x(02)00081-6

Published

2002-05-06

Pages

1975-9

Language

English

Region

Netherlands

NLM ID

8406899

Subset

External Links

PubMed source DOI: 10.1016/s0264-410x(02)00081-6 View journal details

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Muscle specific versus ubiquitous expression of Gag based HIV-1 DNA vaccines: a comparative analysis.

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