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PMID: 11976141 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genomic sequence and evolution of marine cyanophage P60: a new insight on lytic and lysogenic phages.

Applied and environmental microbiology ·Vol. 68 ·No. 5 ·2002-05-00 ·Pages 2589-94

Chen F, Lu J

Abstract

The genome of cyanophage P60, a lytic virus which infects marine Synechococcus WH7803, was completely sequenced. The P60 genome contained 47,872 bp with 80 potential open reading frames that were mostly similar to the genes found in lytic phages like T7, phi-YeO3-12, and SIO1. The DNA replication system, consisting of primase-helicase and DNA polymerase, appeared to be more conserved in podoviruses than in siphoviruses and myoviruses, suggesting that DNA replication genes could be the critical elements for lytic phages. Strikingly high sequence similarities in the regions coding for nucleotide metabolism were found between cyanophage P60 and marine unicellular cyanobacteria.

MeSH Terms
Bacteriophages/classification,genetics,ultrastructure Biological Evolution Cloning, Molecular DNA, Viral/analysis Genome, Viral Marine Biology Microscopy, Electron Molecular Sequence Data Phylogeny Sequence Analysis, DNA
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chen Feng
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202, USA. chenf@umbi.umd.edu
Lu Jingrang
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.68.5.2589-2594.2002
Email
chenf@umbi.umd.edu
Published
2002-05-00
Pages
2589-94
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC127578
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.68.5.2589-2594.2002 PMC Full Text View journal details
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