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PMID: 11970962 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

ARF-GAP-mediated interaction between the ER-Golgi v-SNAREs and the COPI coat.

The Journal of cell biology ·Vol. 157 ·No. 3 ·2002-04-29 ·Pages 395-404

Rein U, Andag U, Duden R, Schmitt HD, Spang A

Abstract

In eukaryotic cells, secretion is achieved by vesicular transport. Fusion of such vesicles with the correct target compartment relies on SNARE proteins on both vesicle (v-SNARE) and the target membranes (t-SNARE). At present it is not clear how v-SNAREs are incorporated into transport vesicles. Here, we show that binding of ADP-ribosylation factor (ARF)-GTPase-activating protein (GAP) to ER-Golgi v-SNAREs is an essential step for recruitment of Arf1p and coatomer, proteins that together form the COPI coat. ARF-GAP acts catalytically to recruit COPI components. Inclusion of v-SNAREs into COPI vesicles could be mediated by direct interaction with the coat. The mechanisms by which v-SNAREs interact with COPI and COPII coat proteins seem to be different and may play a key role in determining specificity in vesicle budding.

MeSH Terms
ADP-Ribosylation Factor 1/metabolism ADP-Ribosylation Factors/metabolism COP-Coated Vesicles/metabolism Coat Protein Complex I/metabolism DNA-Binding Proteins/metabolism Endoplasmic Reticulum/metabolism Fungal Proteins/metabolism GTPase-Activating Proteins/metabolism Intracellular Membranes/metabolism Membrane Proteins/metabolism Microsomes/metabolism Protein Binding Recombinant Fusion Proteins/metabolism SNARE Proteins Saccharomyces cerevisiae Proteins Vesicular Transport Proteins
Chemicals
Coat Protein Complex I DNA-Binding Proteins Fungal Proteins GCS1 protein, S cerevisiae GTPase-Activating Proteins Membrane Proteins Recombinant Fusion Proteins SNARE Proteins Saccharomyces cerevisiae Proteins Vesicular Transport Proteins ADP-Ribosylation Factor 1 ADP-Ribosylation Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rein Ulrike
Friedrich Miescher Laboratory, Max Planck Society, D-72076 Tübingen, Germany.
Andag Uwe
Duden Rainer
Schmitt Hans Dieter
Spang Anne
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200112092
Published
2002-04-29
Epub
2002-00-22
Pages
395-404
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2173288
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1083/jcb.200112092 PMC Full Text View journal details
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