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PMID: 11951978 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

High pressure matrix-assisted laser desorption/ionization Fourier transform mass spectrometry for minimization of ganglioside fragmentation.

Journal of the American Society for Mass Spectrometry ·Vol. 13 ·No. 4 ·2002-04-00 ·Pages 402-7

O'Connor PB, Mirgorodskaya E, Costello CE

Abstract

Transiently elevating pressure in a matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS) source into the 1-10 mbar range during ionization decreases the metastable fragmentation of gangliosides. This allows detection of the molecular ion species without loss of the highly labile sialic acid residues. In these experiments, gangliosides with up to five sialic acids were ionized by MALDI and detected with the FTMS. In each case, when the high pressure collisional cooling was used, the singly charged molecular ion was the base peak in the spectra, both in the positive and negative ion modes, and minimal metastable fragmentation was observed. This result is promising, as the previously developed TLC separation methods can be coupled to MALDI-FTMS.

MeSH Terms
Calibration Chromatography, Thin Layer Fourier Analysis Gangliosides/chemistry Glycolipids/chemistry Sialic Acids/chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Chemicals
Gangliosides Glycolipids Sialic Acids
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
O'Connor Peter B
Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118, USA. poconnor@bu.edu
Mirgorodskaya Ekaterina
Costello Catherine E
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Article Info
Journal
Journal of the American Society for Mass Spectrometry
Abbr.
J Am Soc Mass Spectrom
ISSN
1044-0305
DOI
10.1016/S1044-0305(02)00351-3
Email
poconnor@bu.edu
Published
2002-04-00
Pages
402-7
Language
English
Region
United States
NLM ID
9010412
Subset
IM
Grants
NCRR NIH HHS · P41-RR10888 · United States
External Links
PubMed source DOI: 10.1016/S1044-0305(02)00351-3 View journal details
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