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PMID: 11948172 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

ZipA is required for recruitment of FtsK, FtsQ, FtsL, and FtsN to the septal ring in Escherichia coli.

Journal of bacteriology ·Vol. 184 ·No. 9 ·2002-05-00 ·Pages 2552-6

Hale CA, de Boer PA

Abstract

The septal ring in Escherichia coli consists of at least nine essential gene products whose order of assembly resembles a mostly linear dependency pathway: FtsA and ZipA directly bind FtsZ polymers at the prospective division site, followed by the sequential addition of FtsK, FtsQ, FtsL, FtsW, FtsI, and FtsN. Recruitment of FtsK and all downstream components requires the prior localization of FtsA. Here we show that recruitment of FtsK, FtsQ, FtsL, and FtsN equally requires ZipA. The results imply that association of both FtsA and ZipA with FtsZ polymers is needed for further maturation of the nascent organelle.

MeSH Terms
Bacterial Proteins/metabolism Carrier Proteins/metabolism,physiology Cell Cycle Proteins/metabolism,physiology Cell Division Escherichia coli/cytology,metabolism,physiology Escherichia coli Proteins Membrane Proteins/metabolism Microscopy, Fluorescence Organelles
Chemicals
Bacterial Proteins Carrier Proteins Cell Cycle Proteins Escherichia coli Proteins FtsK protein, E coli FtsN protein, E coli FtsQ protein, E coli Membrane Proteins ZipA protein, E coli ftsL protein, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hale Cynthia A
Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4960, USA. cah19@po.cwru.edu
de Boer Piet A J
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28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.184.9.2552-2556.2002
Email
cah19@po.cwru.edu
Published
2002-05-00
Pages
2552-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC135003
Subset
IM
Grants
NIGMS NIH HHS · R01 GM057059 · United States
NIGMS NIH HHS · GM-57059 · United States
External Links
PubMed source DOI: 10.1128/JB.184.9.2552-2556.2002 PMC Full Text View journal details
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