Production, purification, and chemical characterization of Staphylococcus aureus exfoliative toxin.

Infection and immunity ·Vol. 12 ·No. 5 ·1975-11-00 ·Pages 1206-10

Johnson AD, Metzger JF, Spero L

Abstract

Methods for the production and isolation of exfoliative toxin are described. Fermentation conditions were established under which large quantities of the crude material can be produced. Column chromatography methods, including carboxymethyl cellulose and hydroxyapatite, were utilized to purify the toxic protein. The pure toxin had a molecular weight of 26,000 as determined by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The pure toxin is a simple protein composed of 17 amino acids. Tests for carbohydrate and for alpha- and beta-hemolysin were negative. The mean effective dose of the purified toxin was 0.5 mug per newborn mouse.

MeSH Terms

Amino Acids/analysis Bacterial Proteins/analysis,biosynthesis,isolation & purification Electrophoresis, Polyacrylamide Gel Fermentation Nitrogen/analysis Staphylococcus aureus Toxins, Biological/analysis,biosynthesis,isolation & purification

Chemicals

Amino Acids Bacterial Proteins Toxins, Biological Nitrogen

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Johnson A D

Metzger J F

Spero L

References (12)

12 references, click to expand

The action of carboxypeptidases A and B on the separated alpha and beta chains of normal adult human hemoglobin.
Biochim Biophys Acta. 1960 Jul 29;42:177-9 PMID: 13709581
Purification of exfoliatin produced by Staphylococcus aureus of bacteriophage group 2 and its physicochemical properties.
Infect Immun. 1973 Aug;8(2):156-64 PMID: 4269383
The staphylococcal scalded-skin syndrome: isolation and partial characterization of the exfoliative toxin.
J Infect Dis. 1972 Feb;125(2):129-40 PMID: 4550227
Product of Staphylococcus aureus responsible for the scalded-skin syndrome.
Infect Immun. 1971 Nov;4(5):541-5 PMID: 5154894
[On the determination of the most coherent molecular weight with analysis of the amino acids of a protein].
Biochim Biophys Acta. 1968 Dec 3;168(3):573-5 PMID: 5701717
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
Spectroscopic determination of tryptophan and tyrosine in proteins.
Biochemistry. 1967 Jul;6(7):1948-54 PMID: 6049437
Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion.
Acta Pathol Microbiol Scand. 1953;32(2):230-40 PMID: 13079779
Interaction of staphylococcal exfoliative toxin with concanavalin A.
Infect Immun. 1974 Dec;10(6):1260-5 PMID: 4215760
The gas chromatographic properties of biologically important N-acetylglucosamine derivatives, monosaccharides, disaccharides, trisaccharides, tetrasaccharides and pentasaccharides.
Biochim Biophys Acta. 1970 Nov 24;222(2):339-47 PMID: 5491218
The scalded skin syndrome in small children.
Arch Dermatol. 1967 Apr;95(4):359-69 PMID: 6023040
The estimation of threonine and serine in proteins.
Biochem J. 1946;40(5-6):632-40 PMID: 16748066

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.12.5.1206-1210.1975

Published

1975-11-00

Pages

1206-10

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC415417

Subset

External Links

PubMed source DOI: 10.1128/iai.12.5.1206-1210.1975 PMC Full Text View journal details

Analysis Services

Production, purification, and chemical characterization of Staphylococcus aureus exfoliative toxin.

Contact

WeChat Official Account