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PMID: 11918087 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Monocyte chemoattractant protein-1 (MCP-1), secreted by bone marrow endothelial cells, induces chemoattraction of 5T multiple myeloma cells.

Clinical & experimental metastasis ·Vol. 19 ·No. 1 ·2002-00-00 ·Pages 87-90

Vanderkerken K, Vande Broek I, Eizirik DL, Van Valckenborgh E, Asosingh K, Van Riet I, Van Camp B

Abstract

Homing of multiple myeloma (MM) cells to the bone marrow (BM) requires transendothelial migration. In the present work we tested whether monocyte chemoattractant protein-1 (MCP-1) and CCR2, the high affinity receptor for MCP-1, are involved in this process. Murine 5T2 and 5T33MM cell lines were selected as source of MM cells and STR4, 10 and 12 of BM endothelial cells (BMEC). RT-PCR demonstrated transcripts for MCP-1 in BMEC and ELISA the presence of MCP-1 protein in culture medium. RNase protection assay showed mRNA expression for CCR2, and FACS analysis the presence of CCR2 protein on the MM cells. EC conditioned medium induced chemoattraction of MM cells, a phenomenon inhibited by anti-MCP-1 antibodies. In conclusion, MM cells express CCR2 and are attracted by MCP-1 secreted by BMEC. We suggest that local MCP-1 production by BMEC is one of the mechanisms involved in homing of myeloma cells to the BM.

MeSH Terms

Animals Bone Marrow/metabolism Cell Line, Transformed Chemokine CCL2/metabolism,pharmacology Chemotaxis/drug effects Endothelium/metabolism,pathology Mice Mice, Inbred C57BL Multiple Myeloma/genetics,pathology Neoplasm Proteins/drug effects,genetics,physiology RNA, Messenger/analysis RNA, Neoplasm/analysis Receptors, CCR2 Receptors, Chemokine/drug effects,genetics,physiology

Chemicals

Ccr2 protein, mouse Chemokine CCL2 Neoplasm Proteins RNA, Messenger RNA, Neoplasm Receptors, CCR2 Receptors, Chemokine

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Vanderkerken Karin

Department of Hematology and Immunology, Free University Brussels (VUB), Belgium. Karin.Vanderkerken@vub.ac.be

Vande Broek Isabelle

Eizirik Décio L

Van Valckenborgh Els

Asosingh Kewal

Van Riet Ivan

Van Camp Ben

References (13)

13 references, click to expand

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Article Info

Journal

Clinical & experimental metastasis

Abbr.

Clin Exp Metastasis

ISSN

0262-0898

DOI

10.1023/a:1013891205989

karin.vanderkerken@vub.ac.be

Published

2002-00-00

Pages

87-90

Language

English

Region

Netherlands

NLM ID

8409970

Subset

External Links

PubMed source DOI: 10.1023/a:1013891205989 View journal details

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Monocyte chemoattractant protein-1 (MCP-1), secreted by bone marrow endothelial cells, induces chemoattraction of 5T multiple myeloma cells.

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