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PMID: 11895993 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Membrane localization contributes to the in vivo ADP-ribosylation of Ras by Pseudomonas aeruginosa ExoS.

Infection and immunity ·Vol. 70 ·No. 4 ·2002-04-00 ·Pages 2230-2

Riese MJ, Barbieri JT

Abstract

Type III-delivered exoenzyme S (ExoS) preferentially ADP-ribosylated membrane-associated His(6)HRas, relative to its cytosolic derivative His(6)HRas Delta CAAX. This indicates that the subcellular protein distribution contributes to in vivo ADP-ribosylation by ExoS.

MeSH Terms

ADP Ribose Transferases/metabolism Adenosine Diphosphate Ribose/metabolism Animals Bacterial Toxins CHO Cells Cell Membrane/metabolism Cricetinae Pseudomonas aeruginosa/enzymology ras Proteins/metabolism

Chemicals

Bacterial Toxins Adenosine Diphosphate Ribose ADP Ribose Transferases exoenzyme S ras Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Riese Matthew J

Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA.

Barbieri Joseph T

References (13)

13 references, click to expand

Targeting of the Yersinia pestis YopM protein into HeLa cells and intracellular trafficking to the nucleus.
Mol Microbiol. 1998 Dec;30(5):1051-65 PMID: 9988481
Endomembrane trafficking of ras: the CAAX motif targets proteins to the ER and Golgi.
Cell. 1999 Jul 9;98(1):69-80 PMID: 10412982
Pseudomonas aeruginosa exoenzyme S disrupts Ras-mediated signal transduction by inhibiting guanine nucleotide exchange factor-catalyzed nucleotide exchange.
J Biol Chem. 1999 Jul 30;274(31):21823-9 PMID: 10419499
The N-terminal domain of Pseudomonas aeruginosa exoenzyme S is a GTPase-activating protein for Rho GTPases.
J Biol Chem. 1999 Dec 17;274(51):36369-72 PMID: 10593930
Intracellular localization and processing of Pseudomonas aeruginosa ExoS in eukaryotic cells.
Mol Microbiol. 2000 Jul;37(2):287-99 PMID: 10931325
Pseudomonas aeruginosa exoenzyme S, a bifunctional type-III secreted cytotoxin.
Int J Med Microbiol. 2000 Oct;290(4-5):381-7 PMID: 11111915
Intracellular expression of the ADP-ribosyltransferase domain of Pseudomonas exoenzyme S is cytotoxic to eukaryotic cells.
Mol Microbiol. 1998 Nov;30(4):751-9 PMID: 10094623
Independent and coordinate effects of ADP-ribosyltransferase and GTPase-activating activities of exoenzyme S on HT-29 epithelial cell function.
Infect Immun. 2001 Sep;69(9):5318-28 PMID: 11500401
Auto-ADP-ribosylation of Pseudomonas aeruginosa ExoS.
J Biol Chem. 2002 Apr 5;277(14):12082-8 PMID: 11821389
Several GTP-binding proteins, including p21c-H-ras, are preferred substrates of Pseudomonas aeruginosa exoenzyme S.
J Biol Chem. 1989 May 25;264(15):9004-8 PMID: 2498323
Exocytosis from permeabilized bovine adrenal chromaffin cells is differently modulated by guanosine 5'-[gamma-thio]triphosphate and guanosine 5'-[beta gamma-imido]triphosphate. Evidence for the involvement of various guanine nucleotide-binding proteins.
Biochem J. 1992 Jun 1;284 ( Pt 2):321-6 PMID: 1599416
Modification of Ras in eukaryotic cells by Pseudomonas aeruginosa exoenzyme S.
Infect Immun. 1998 Jun;66(6):2607-13 PMID: 9596723
ADP ribosylation of Arg41 of Rap by ExoS inhibits the ability of Rap to interact with its guanine nucleotide exchange factor, C3G.
Biochemistry. 2001 Mar 20;40(11):3289-94 PMID: 11258948

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/IAI.70.4.2230-2232.2002

Published

2002-04-00

Pages

2230-2

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC127869

Subset

Grants

NIAID NIH HHS · AI-31062 · United States

External Links

PubMed source DOI: 10.1128/IAI.70.4.2230-2232.2002 PMC Full Text View journal details

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Membrane localization contributes to the in vivo ADP-ribosylation of Ras by Pseudomonas aeruginosa ExoS.

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