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PMID: 11884684 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response.

The Plant cell ·Vol. 14 ·No. 2 ·2002-02-00 ·Pages 421-33

del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M

Abstract

Mutations in the AXR1 gene result in a reduction in auxin response and diverse defects in auxin-regulated growth and development. In a previous study, we showed that AXR1 forms a heterodimer with the ECR1 protein. This enzyme activates the ubiquitin-related protein RUB1 in vitro. Furthermore, we showed that the Skp1-Cul1/Cdc53-F-box (SCF) subunit AtCUL1 is modified by RUB1 in vivo. In this report, we demonstrate that the formation of RUB-AtCUL1 is dependent on AXR1 and ECR1 in vivo. The expression of AXR1 and ECR1 is restricted to zones of active cell division and cell elongation, consistent with their role in growth regulation. These results provide strong support for a model in which RUB conjugation of AtCUL1 affects the function of SCF E3s that are required for auxin response.

MeSH Terms
2,4-Dichlorophenoxyacetic Acid/pharmacology Arabidopsis/drug effects,genetics,growth & development Arabidopsis Proteins/genetics,metabolism Cell Nucleus/metabolism Gene Expression Regulation, Plant/drug effects Growth Substances Indoleacetic Acids/pharmacology Meristem/genetics,metabolism Mutation Peptide Synthases/genetics Plant Proteins/genetics SKP Cullin F-Box Protein Ligases Ubiquitins
Chemicals
AXR1 protein, Arabidopsis Arabidopsis Proteins ECR1 protein, Arabidopsis Growth Substances Indoleacetic Acids Plant Proteins RUB1 protein, Arabidopsis Ubiquitins 2,4-Dichlorophenoxyacetic Acid SKP Cullin F-Box Protein Ligases Peptide Synthases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
del Pozo Juan C
Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Texas 78712, USA.
Dharmasiri Sunethra
Hellmann Hanjo
Walker Loni
Gray William M
Estelle Mark
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.010282
Published
2002-02-00
Pages
421-33
Language
English
Region
England
NLM ID
9208688
PMCID
PMC152922
Subset
IM
Grants
NIGMS NIH HHS · R01 GM043644 · United States
NIGMS NIH HHS · R01-GM43644 · United States
External Links
PubMed source DOI: 10.1105/tpc.010282 PMC Full Text View journal details
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