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PMID: 11872120 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genetic diversity of vaginal lactobacilli from women in different countries based on 16S rRNA gene sequences.

Journal of applied microbiology ·Vol. 92 ·No. 3 ·2002-00-00 ·Pages 451-9

Pavlova SI, Kilic AO, Kilic SS, So JS, Nader-Macias ME, Simoes JA, Tao L

Abstract

Lactobacilli are widely distributed in food and the environment, and some colonize the human body as commensal bacteria. The aim of this study was to determine the species of lactobacilli that colonize the vagina and compare them with those found in food and the environment. Thirty-five Lactobacillus strains from women from seven countries were isolated, and sequences from 16S rRNA genes were determined and compared with existing data in GenBank. A phylogenetic tree was achieved using the Neighbour-Joining method based on the analysis of 1465 nucleotides. The results showed that most vaginal isolates were L. crispatus, L. jensenii and L. gasseri. Some were L. vaginalis, L. fermentum, L. mucosae, L. paracasei and L. rhamnosus. Two isolates from a native American woman displayed distinct branches, indicating novel phylotypes. Few vaginal isolates matched food or environmental Lactobacillus species. Most women worldwide were colonized by three common Lactobacillus species: L. crispatus, L. jensenii and L. gasseri. Knowledge of vaginal Lactobacillus species richness and distribution in women worldwide may lead to the design of better probiotic products as bacterial replacement therapy.

MeSH Terms

Adult DNA, Ribosomal/analysis Female Genetic Variation Global Health Humans Lactobacillus/classification,genetics Middle Aged Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Vagina/microbiology

Chemicals

DNA, Ribosomal RNA, Ribosomal, 16S

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Pavlova S I

Department of Oral Biology, College of Destistry, University of Illinois at Chicago, M/C 690, 801 South Paulina Street, Chicago, IL 60612, USA.

Kilic A O

Kilic S S

So J-S

Nader-Macias M E

Simoes J A

Tao L

Article Info

Journal

Journal of applied microbiology

Abbr.

J Appl Microbiol

ISSN

1364-5072

DOI

10.1046/j.1365-2672.2002.01547.x

Published

2002-00-00

Pages

451-9

Language

English

Region

England

NLM ID

9706280

Subset

Grants

PHS HHS · R03 A1 45127 · United States

Databases

GENBANK

AF243141, AF243142, AF243143, AF243144, AF243145, AF243146, AF243147, AF243148, AF243149, AF243150, AF243151, AF243152, AF243153, AF243154, AF243155, AF243156, AF243157, AF243158, AF243159, AF243160, AF243161, AF243162, AF243163, AF243164, AF243165, AF243166, AF243167, AF243168, AF243169, AF243170, AF243171, AF243172, AF243173, AF243174, AF243175, AF243176, AF243177, AF257096, AF257097

External Links

PubMed source DOI: 10.1046/j.1365-2672.2002.01547.x View journal details

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Genetic diversity of vaginal lactobacilli from women in different countries based on 16S rRNA gene sequences.

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