| GeneLibs

题目:: SV40 T antigen and telomerase are required to obtain immortalized human adult bone cells without loss of the differentiated phenotype.
作者:: Darimont(Christian),Avanti(Ornella),Tromvoukis(Yvonne),Vautravers-Leone(Patricia),Kurihara(Nori),Roodman(G David),Colgin(Lorel M),Tullberg-Reinert(Heide),Pfeifer(Andrea M A),Offord(Elizabeth A),Mace(Katherine)
状态:: 发布时间2002-02-26 , 更新时间 2006-11-15
期刊:: Cell Growth Differ
摘要:: In most human primary bone cells, SV40 T-antigen expression was able to expand life span for a few passages before cells undergo growth arrest, described as crisis. In this study, telomerase activity was reconstituted in human osteoblast precursors (hPOB cells) and marrow stromal cells (Saka cells) transformed with the SV40 T antigen. Bone cells with telomerase activity were able to bypass crisis and show unlimited life span. Despite chromosomal aberrations observed in hPOB-tert cells, these immortalized precursors were able to differentiate into osteoblasts like precrisis hPOB cells. Saka-tert cells enhanced the formation of human osteoclast-like cells in a similar manner as Saka cells. These results demonstrate that reconstitution of telomerase activity in transformed SV40 T-antigen human osteoblast precursors or marrow stromal cells leads to the generation of immortalized cells with a preserved phenotype.
语言:: eng
DOI:

文献库文献相关信息