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PMID: 11861551 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Schizosaccharomyces pombe Bir1p, a nuclear protein that localizes to kinetochores and the spindle midzone, is essential for chromosome condensation and spindle elongation during mitosis.

Genetics ·Vol. 160 ·No. 2 ·2002-02-00 ·Pages 445-56

Rajagopalan S, Balasubramanian MK

Abstract

The inhibitor of apoptosis (IAP) family of proteins contains a subset of members characterized by the presence of highly conserved baculoviral IAP repeat (BIR) domains. Recent work has shown that some of these BIR-domain proteins play a prominent role in the regulation of cell division, in particular at the stage of chromosome segregation and cytokinesis. We and others have shown that the Schizosaccharomyces pombe BIR-domain protein, Bir1p/Pbh1p/Cut17p, is important for the regulation of mitosis. Here we further characterize S. pombe Bir1p using methods of cell biology and genetics. We show that Bir1p is dispersed throughout the nucleus during the cell cycle. In addition, a significant part of Bir1p is also detected at the kinetochores and the spindle midzone during mitosis and meiosis. Time-lapse microscopy studies suggest that Bir1p relocates from the kinetochores to the spindle at the end of anaphase A. Bir1p colocalizes with the S. pombe Aurora kinase homolog Aim1p, a protein essential for mitosis, at the kinetochores as well as the spindle midzone during mitosis, and functional Bir1p is essential for localization of Aim1p to the kinetochores and the spindle midzone. Analyses of bir1 conditional mutants revealed that Bir1p is essential for chromosome condensation during mitosis. In addition, anaphase cells show the presence of lagging chromosomes and a defect in spindle elongation. We conclude that Bir1p is important for multiple processes that occur during mitosis in S. pombe.

MeSH Terms
Anaphase/physiology Cell Cycle Proteins/genetics,physiology Cell Nucleus/physiology,ultrastructure Fungal Proteins/genetics,physiology Kinetochores/physiology,ultrastructure Mutation Schizosaccharomyces/cytology,genetics,physiology Schizosaccharomyces pombe Proteins Spindle Apparatus/physiology,ultrastructure Survivin
Chemicals
Bir1 protein, S pombe Cell Cycle Proteins Fungal Proteins Schizosaccharomyces pombe Proteins Survivin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rajagopalan Srividya
Cell Division Laboratory, The Institute of Molecular Agrobiology, The National University of Singapore, 117604 Singapore.
Balasubramanian Mohan K
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/160.2.445
Published
2002-02-00
Pages
445-56
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1461985
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/160.2.445 PMC Full Text View journal details
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