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PMID: 11854224 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The sortase SrtA of Listeria monocytogenes is involved in processing of internalin and in virulence.

Infection and immunity ·Vol. 70 ·No. 3 ·2002-03-00 ·Pages 1382-90

Garandeau C, Réglier-Poupet H, Dubail I, Beretti JL, Berche P, Charbit A

Abstract

Listeria monocytogenes is an intracellular gram-positive human pathogen that invades eucaryotic cells. Among the surface-exposed proteins playing a role in this invasive process, internalin belongs to the family of LPXTG proteins, which are known to be covalently linked to the bacterial cell wall in gram-positive bacteria. Recently, it has been shown in Staphylococcus aureus that the covalent anchoring of protein A, a typical LPXTG protein, is due to a cysteine protease, named sortase, required for bacterial virulence. Here, we identified in silico from the genome of L. monocytogenes a gene, designated srtA, encoding a sortase homologue. The role of this previously unknown sortase was studied by constructing a sortase knockout mutant. Internalin was used as a reporter protein to study the effects of the srtA mutation on cell wall anchoring of this LPXTG protein in L. monocytogenes. We show that the srtA mutant (i) is affected in the display of internalin at the bacterial surface, (ii) is significantly less invasive in vitro, and (iii) is attenuated in its virulence in the mouse. These results demonstrate that srtA of L. monocytogenes acts as a sortase and plays a role in the pathogenicity.

MeSH Terms
Amino Acid Sequence Aminoacyltransferases/genetics,metabolism Animals Bacterial Proteins/metabolism Cysteine Endopeptidases Female Genes, Bacterial Genome, Bacterial Listeria monocytogenes/genetics,metabolism,pathogenicity Mice Molecular Sequence Data Protein Binding Protein Processing, Post-Translational Protein Transport Sequence Analysis, DNA Sequence Homology, Amino Acid Solubility
Chemicals
Bacterial Proteins internalin protein, Bacteria Aminoacyltransferases sortase A Cysteine Endopeptidases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Garandeau Caroline
INSERM U-411, CHU Necker-Enfants Malades, 75730 Paris Cedex 15, France.
Réglier-Poupet Hélène
Dubail Iharilalao
Beretti Jean-Luc
Berche Patrick
Charbit Alain
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
Published
2002-03-00
Pages
1382-90
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC127754
Subset
IM
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