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PMID: 11847110 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ca(2+) dynamics in the lumen of the endoplasmic reticulum in sensory neurons: direct visualization of Ca(2+)-induced Ca(2+) release triggered by physiological Ca(2+) entry.

The EMBO journal ·Vol. 21 ·No. 4 ·2002-02-15 ·Pages 622-30

Solovyova N, Veselovsky N, Toescu EC, Verkhratsky A

Abstract

In cultured rat dorsal root ganglia neurons, we measured membrane currents, using the patch-clamp whole-cell technique, and the concentrations of free Ca(2+) in the cytosol ([Ca(2+)](i)) and in the lumen of the endoplasmic reticulum (ER) ([Ca(2+)](L)), using high- (Fluo-3) and low- (Mag-Fura-2) affinity Ca(2+)-sensitive fluorescent probes and video imaging. Resting [Ca(2+)](L) concentration varied between 60 and 270 microM. Activation of ryanodine receptors by caffeine triggered a rapid fall in [Ca(2+)](L) levels, which amounted to only 40--50% of the resting [Ca(2+)](L) value. Using electrophysiological depolarization, we directly demonstrate the process of Ca(2+)-induced Ca(2+) release triggered by Ca(2+) entry through voltage-gated Ca(2+) channels. The amplitude of Ca(2+) release from the ER lumen was linearly dependent on I(Ca).

MeSH Terms
Animals Caffeine/pharmacology Calcium/metabolism,physiology Calcium Signaling Endoplasmic Reticulum/drug effects,metabolism Male Neurons, Afferent/drug effects,metabolism Rats Rats, Sprague-Dawley Ryanodine Receptor Calcium Release Channel/drug effects,metabolism
Chemicals
Ryanodine Receptor Calcium Release Channel Caffeine Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Solovyova N
The University of Manchester, School of Biological Sciences, 1.124 Stopford Building, Oxford Road, Manchester M13 9PT, UK.
Veselovsky N
Toescu E C
Verkhratsky A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/21.4.622
Published
2002-02-15
Pages
622-30
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125857
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/21.4.622 PMC Full Text View journal details
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