Home LiteratureArticle Details
PMID: 1182580 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The metabolism of labeled parathyroid hormone. II. Methodological studies.

Calcified tissue research ·Vol. 18 ·No. 4 ·1975-09-17 ·Pages 241-50

Neuman WF, Neuman MW, Sammon PJ, Lane K

Abstract

Further studies on the preparation of 125I-labeled, biologically active parathyroid hormone have resulted in improvements in the procedure, in the storage of the labeled product, and in the establisment of the location of the label in the polypeptide chain. Approximately 95% of the label is on the single tyrosyl residue, amino acid 43, in the bovine hormone. New procedures have also been developed for the isolation and quantitative gel filtration of the labeled hormone deposited in tissues (liver, kidney and bone).

MeSH Terms
Amino Acid Sequence Animals Bone and Bones/metabolism Cattle Chromatography, Gel/methods Drug Stability Drug Storage Electrolysis Iodine Radioisotopes Isotope Labeling/methods Kidney/metabolism Liver/metabolism Molecular Weight Parathyroid Hormone/isolation & purification,metabolism Tyrosine/metabolism
Chemicals
Iodine Radioisotopes Parathyroid Hormone Tyrosine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Neuman W F
Neuman M W
Sammon P J
Lane K
References (11)
11 references, click to expand
  1. Metabolism of labeled parathyroid hormone. I. Preparation of biologically active I-125-labeled parathyroid hormone.
    Endocrinology. 1973 Jun;92(6):1596-603 PMID: 4707255
  2. Parathyroid hormone: sequence, synthesis, immunoassay studies.
    Am J Med. 1971 May;50(5):639-49 PMID: 5575546
  3. The tissue localization of tritiated parathyroid hormone in thyroparathyroidectomized rats.
    J Biol Chem. 1972 Apr 10;247(7):2195-9 PMID: 5016649
  4. The metabolism of labeled parathyroid hormone. III. Studies in rats.
    Calcif Tissue Res. 1975 Sep 17;18(4):251-61 PMID: 1182581
  5. Preparation of radioiodinated insulin by constant current electrolysis.
    J Nucl Biol Med. 1969 Jan-Jun;13(1):64-70 PMID: 5383775
  6. Synthesis and secretion of parathyroid hormone in vitro.
    Am J Med. 1971 May;50(5):658-69 PMID: 5575548
  7. Parathyroid hormone: secretion and metabolism in vivo.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):2986-91 PMID: 5289243
  8. Evidence for a precursor to circulating parathyroid hormone.
    Proc Natl Acad Sci U S A. 1970 Nov;67(3):1631-8 PMID: 5274485
  9. Human parathyroid hormone: glandular and secreted molecular species.
    Am J Med. 1971 May;50(5):630-8 PMID: 5575545
  10. Nonenzymatic cleavage of peptide bonds: the methionine residues in bovine pancreatic ribonuclease.
    J Biol Chem. 1962 Jun;237:1856-60 PMID: 13902203
  11. Native human parathyroid hormone: an immunochemical investigation.
    Proc Natl Acad Sci U S A. 1970 Sep;67(1):415-22 PMID: 5272323
Article Info
Journal
Calcified tissue research
Abbr.
Calcif Tissue Res
ISSN
0008-0594
DOI
10.1007/BF02546243
Published
1975-09-17
Pages
241-50
Language
English
Region
Germany
NLM ID
0114414
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02546243
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2