Significance of the detection of beta-galactosidase and of beta-xylosidase in the taxonomic study of the genus Haemophilus.
A total of 314 strains of Haemophilus, isolated from clinical samples, were studied for the production of beta-galactosidase and beta-xylosidase. None of the H. influenzae strains studied (9 beta-lactamase positive strains and 129 beta-lactamase negative strains) possessed these enzymes. Both enzymes were almost constantly observed among strains of H. paraphrophilus (10 strains studied) and of H. paraphrohaemolyticus (9 strains studied). Among the other species (H. parainfluenzae, 55 strains; H. haemolyticus, 5 strains; H. parahaemolyticus, 97 strains), beta-galactosidase was present in about 30% of the strains studied whereas beta-xylosidase was detected occasionally (3% of the strains studied). Detection of these two enzymes could be a valuable test for the taxonomic study of the genus Haemophilus. However, the type of substrate used for the detection of beta-xylosidase is important: use of the para-nitro-phenyl-beta-xylopyranoside yielded more positive results than the use of its ortho-isomer.
13 references, click to expand
-
Rapid detection of ampicillin-resistant Haemophilus influenzae and their susceptibility to sixteen antibiotics.Antimicrob Agents Chemother. 1975 Jul;8(1):91-4 PMID: 1080656
-
The taxonomy of haemophili isolated from conjunctivae.Acta Pathol Microbiol Scand B. 1976 Jun;84(3):132-8 PMID: 1087099
-
Taxonomy of Acinetobacter: the usefulness of beta-D-xyloside xylohydrolase for strain differentiation.J Clin Pathol. 1977 Sep;30(9):838-41 PMID: 410848
-
Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.Antimicrob Agents Chemother. 1972 Apr;1(4):283-8 PMID: 4208895
-
[Taxonomic value of beta xylosidase detection in Enterobacteriaceae].Ann Inst Pasteur (Paris). 1972 Sep;123(3):341-7 PMID: 4565365
-
The ortho-nitrophenol (ONPG) test and acid from lactose in Gram-negative genera.J Clin Pathol. 1973 Nov;26(11):821-5 PMID: 4587939
-
[Antigenic and biochemical study of 500 Klebsiella strains].Ann Biol Clin (Paris). 1973;31(4):295-303 PMID: 4723410
-
[Selective medium for the isolation of Haemophilus in pathological material of the respiratory tract].Ann Biol Clin (Paris). 1973;31(1):47-51 PMID: 4735676
-
A new V-dependent Haemophilus species preferring increased CO2 tension for growth and named Haemophilus paraphrophilus, nov. sp.J Pathol Bacteriol. 1968 Oct;96(2):413-9 PMID: 4880694
-
A haemolytic V-dependent CO2-preferring Haemophilus species (Haemophilus paraphrohaemolyticus nov. spec.).J Med Microbiol. 1971 Feb;4(1):139-43 PMID: 5548598
-
A taxonomic study of the genus Haemophilus, with the proposal of a new species.J Gen Microbiol. 1976 Mar;93(1):9-62 PMID: 772168
-
[Search of some glycosidases in 22 species of (Bacillus) (author's transl)].Ann Microbiol (Paris). 1976 Feb-Mar;127A(2):317-21 PMID: 962252
-
Rapid diagnosis of Enterobacteriaceae. I. Detection of bacterial glycosidases.Acta Pathol Microbiol Scand B. 1976 Oct;84B(5):245-51 PMID: 9774
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