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PMID: 11818335 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genome-wide analysis of mRNAs targeted to yeast mitochondria.

EMBO reports ·Vol. 3 ·No. 2 ·2002-02-00 ·Pages 159-64

Marc P, Margeot A, Devaux F, Blugeon C, Corral-Debrinski M, Jacq C

Abstract

It is agreed that nuclear-encoded mitochondrial proteins are post-translationally targeted to mitochondria, even if, in some cases, a co-translational phase can assist the import of precursor proteins. We used yeast DNA microarrays to analyse the mRNA populations associated with free and mitochondrion-bound polysomes. As expected, many mRNAs, known to encode mitochondrial proteins, are localized to free cytoplasmic polysomes, but many are localized to mitochondrion-bound polysomes. Furthermore, the 3'-UTR of six randomly chosen mitochondrion-bound mRNAs contains sufficient information to target, in vivo, non-translatable RNA to the vicinity of mitochondria. Interestingly, genes producing mRNAs that are targeted to mitochondria are mainly of ancient bacterial origin, whereas those producing mRNAs that are translated in the cytoplasm are mainly of eukaryotic origin. These observations, which support the recent hypotheses concerning the dual origin of the mitochondrial proteome, provide new insights into the biogenesis of mitochondria.

MeSH Terms
DNA, Mitochondrial/genetics Genome, Fungal Oligonucleotide Array Sequence Analysis RNA, Messenger/genetics Saccharomyces cerevisiae/genetics,physiology
Chemicals
DNA, Mitochondrial RNA, Messenger
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Marc Philippe
Laboratoire de Génétique Moléculaire (UMR CNRS 8541), Ecole Normale Supérieure, 46 rue d'Ulm, F-75005 Paris, France.
Margeot Antoine
Devaux Frederic
Blugeon Corinne
Corral-Debrinski Marisol
Jacq Claude
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Article Info
Journal
EMBO reports
Abbr.
EMBO Rep
ISSN
1469-221X
DOI
10.1093/embo-reports/kvf025
Published
2002-02-00
Epub
2002-00-29
Pages
159-64
Language
English
Region
England
NLM ID
100963049
PMCID
PMC1083966
Subset
IM
External Links
PubMed source DOI: 10.1093/embo-reports/kvf025 PMC Full Text View journal details
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