Home LiteratureArticle Details
PMID: 11809814 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Convergence of TOR-nitrogen and Snf1-glucose signaling pathways onto Gln3.

Molecular and cellular biology ·Vol. 22 ·No. 4 ·2002-02-00 ·Pages 1246-52

Bertram PG, Choi JH, Carvalho J, Chan TF, Ai W, Zheng XF

Abstract

Carbon and nitrogen are two basic nutrient sources for cellular organisms. They supply precursors for energy metabolism and metabolic biosynthesis. In the yeast Saccharomyces cerevisiae, distinct sensing and signaling pathways have been described that regulate gene expression in response to the quality of carbon and nitrogen sources, respectively. Gln3 is a GATA-type transcription factor of nitrogen catabolite-repressible (NCR) genes. Previous observations indicate that the quality of nitrogen sources controls the phosphorylation and cytoplasmic retention of Gln3 via the target of rapamycin (TOR) protein. In this study, we show that glucose also regulates Gln3 phosphorylation and subcellular localization, which is mediated by Snf1, the yeast homolog of AMP-dependent protein kinase and a cytoplasmic glucose sensor. Our data show that glucose and nitrogen signaling pathways converge onto Gln3, which may be critical for both nutrient sensing and starvation responses.

MeSH Terms
Cell Cycle Proteins DNA-Binding Proteins/metabolism Fungal Proteins/metabolism Genes, Reporter Glucose/metabolism Nitrogen/metabolism Phosphatidylinositol 3-Kinases Phosphorylation Phosphotransferases (Alcohol Group Acceptor)/metabolism Protein Serine-Threonine Kinases/metabolism Recombinant Fusion Proteins/genetics,metabolism Repressor Proteins Saccharomyces cerevisiae/physiology Saccharomyces cerevisiae Proteins/metabolism Signal Transduction/physiology Transcription Factors/metabolism Two-Hybrid System Techniques
Chemicals
Cell Cycle Proteins DNA-Binding Proteins Fungal Proteins GLN3 protein, S cerevisiae Recombinant Fusion Proteins Repressor Proteins Saccharomyces cerevisiae Proteins Transcription Factors Phosphotransferases (Alcohol Group Acceptor) SNF1-related protein kinases TOR1 protein, S cerevisiae TOR2 protein, S cerevisiae Protein Serine-Threonine Kinases Glucose Nitrogen
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bertram Paula G
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Choi Jae H
Carvalho John
Chan Ting-Fung
Ai Wandong
Zheng X F Steven
References (42)
42 references, click to expand
  1. Target of rapamycin in yeast, TOR2, is an essential phosphatidylinositol kinase homolog required for G1 progression.
    Cell. 1993 May 7;73(3):585-96 PMID: 8387896
  2. Physiological and genetic analysis of the carbon regulation of the NAD-dependent glutamate dehydrogenase of Saccharomyces cerevisiae.
    Mol Cell Biol. 1991 Sep;11(9):4455-65 PMID: 1652057
  3. Yeast TOR (DRR) proteins: amino-acid sequence alignment and identification of structural motifs.
    Gene. 1994 Apr 8;141(1):133-6 PMID: 8163165
  4. Role of the GATA factors Gln3p and Nil1p of Saccharomyces cerevisiae in the expression of nitrogen-regulated genes.
    Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9450-4 PMID: 7568152
  5. Gat1p, a GATA family protein whose production is sensitive to nitrogen catabolite repression, participates in transcriptional activation of nitrogen-catabolic genes in Saccharomyces cerevisiae.
    Mol Cell Biol. 1996 Mar;16(3):847-58 PMID: 8622686
  6. Nutrients, via the Tor proteins, stimulate the association of Tap42 with type 2A phosphatases.
    Genes Dev. 1996 Aug 1;10(15):1904-16 PMID: 8756348
  7. Two different repressors collaborate to restrict expression of the yeast glucose transporter genes HXT2 and HXT4 to low levels of glucose.
    Mol Cell Biol. 1996 Oct;16(10):5536-45 PMID: 8816466
  8. A novel signal transduction pathway in Saccharomyces cerevisiae defined by Snf3-regulated expression of HXT6.
    Mol Biol Cell. 1996 Dec;7(12):1953-66 PMID: 8970157
  9. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications.
    Yeast. 1998 Jan 30;14(2):115-32 PMID: 9483801
  10. The permease homologue Ssy1p controls the expression of amino acid and peptide transporter genes in Saccharomyces cerevisiae.
    Mol Microbiol. 1998 Feb;27(3):643-50 PMID: 9489675
  11. Negative control of the Mig1p repressor by Snf1p-dependent phosphorylation in the absence of glucose.
    Eur J Biochem. 1998 Feb 15;252(1):162-8 PMID: 9523726
  12. Glucose-regulated interaction of a regulatory subunit of protein phosphatase 1 with the Snf1 protein kinase in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1998 May 26;95(11):6245-50 PMID: 9600950
  13. Yeast carbon catabolite repression.
    Microbiol Mol Biol Rev. 1998 Jun;62(2):334-61 PMID: 9618445
  14. The AMP-activated/SNF1 protein kinase subfamily: metabolic sensors of the eukaryotic cell?
    Annu Rev Biochem. 1998;67:821-55 PMID: 9759505
  15. Snf1 protein kinase regulates phosphorylation of the Mig1 repressor in Saccharomyces cerevisiae.
    Mol Cell Biol. 1998 Nov;18(11):6273-80 PMID: 9774644
  16. The 14-3-3 proteins positively regulate rapamycin-sensitive signaling.
    Curr Biol. 1998 Nov 19;8(23):1259-67 PMID: 9822578
  17. Amino acid signaling in Saccharomyces cerevisiae: a permease-like sensor of external amino acids and F-Box protein Grr1p are required for transcriptional induction of the AGP1 gene, which encodes a broad-specificity amino acid permease.
    Mol Cell Biol. 1999 Feb;19(2):989-1001 PMID: 9891035
  18. Target of rapamycin (TOR): balancing the opposing forces of protein synthesis and degradation.
    Curr Opin Genet Dev. 1999 Feb;9(1):49-54 PMID: 10072357
  19. The PIK-related kinases intercept conventional signaling pathways.
    Chem Biol. 1999 May;6(5):R129-36 PMID: 10322127
  20. Glucose repression in yeast.
    Curr Opin Microbiol. 1999 Apr;2(2):202-7 PMID: 10322167
  21. Ssy1p and Ptr3p are plasma membrane components of a yeast system that senses extracellular amino acids.
    Mol Cell Biol. 1999 Aug;19(8):5405-16 PMID: 10409731
  22. Function and regulation of yeast hexose transporters.
    Microbiol Mol Biol Rev. 1999 Sep;63(3):554-69 PMID: 10477308
  23. Nitrogen catabolite repression in Saccharomyces cerevisiae.
    Mol Biotechnol. 1999 Aug;12(1):35-73 PMID: 10554772
  24. The TOR signalling pathway controls nuclear localization of nutrient-regulated transcription factors.
    Nature. 1999 Dec 9;402(6762):689-92 PMID: 10604478
  25. Rapamycin-modulated transcription defines the subset of nutrient-sensitive signaling pathways directly controlled by the Tor proteins.
    Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):14866-70 PMID: 10611304
  26. The TOR signaling cascade regulates gene expression in response to nutrients.
    Genes Dev. 1999 Dec 15;13(24):3271-9 PMID: 10617575
  27. TOR, a central controller of cell growth.
    Cell. 2000 Oct 13;103(2):253-62 PMID: 11057898
  28. A chemical genomics approach toward understanding the global functions of the target of rapamycin protein (TOR).
    Proc Natl Acad Sci U S A. 2000 Nov 21;97(24):13227-32 PMID: 11078525
  29. Tripartite regulation of Gln3p by TOR, Ure2p, and phosphatases.
    J Biol Chem. 2000 Nov 17;275(46):35727-33 PMID: 10940301
  30. Partitioning the transcriptional program induced by rapamycin among the effectors of the Tor proteins.
    Curr Biol. 2000 Dec 14-28;10(24):1574-81 PMID: 11137008
  31. Regulation of APG14 expression by the GATA-type transcription factor Gln3p.
    J Biol Chem. 2001 Mar 2;276(9):6463-7 PMID: 11096087
  32. The TOR kinases link nutrient sensing to cell growth.
    J Biol Chem. 2001 Mar 30;276(13):9583-6 PMID: 11266435
  33. The target of rapamycin (TOR) proteins.
    Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7037-44 PMID: 11416184
  34. Carbon- and nitrogen-quality signaling to translation are mediated by distinct GATA-type transcription factors.
    Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7283-8 PMID: 11416207
  35. Phosphorylation regulates the interaction between Gln3p and the nuclear import factor Srp1p.
    J Biol Chem. 2001 Jul 6;276(27):25359-65 PMID: 11331291
  36. Gln3p nuclear localization and interaction with Ure2p in Saccharomyces cerevisiae.
    J Biol Chem. 2001 Aug 24;276(34):32136-44 PMID: 11408486
  37. A yeast gene that is essential for release from glucose repression encodes a protein kinase.
    Science. 1986 Sep 12;233(4769):1175-80 PMID: 3526554
  38. Regulation of nitrogen assimilation in Saccharomyces cerevisiae: roles of the URE2 and GLN3 genes.
    J Bacteriol. 1988 Feb;170(2):708-13 PMID: 2892826
  39. A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae.
    Nucleic Acids Res. 1990 May 25;18(10):3091-2 PMID: 2190191
  40. Two systems of glucose repression of the GAL1 promoter in Saccharomyces cerevisiae.
    Mol Cell Biol. 1990 Sep;10(9):4757-69 PMID: 2201902
  41. The URE2 gene product of Saccharomyces cerevisiae plays an important role in the cellular response to the nitrogen source and has homology to glutathione s-transferases.
    Mol Cell Biol. 1991 Feb;11(2):822-32 PMID: 1990286
  42. [URE3] as an altered URE2 protein: evidence for a prion analog in Saccharomyces cerevisiae.
    Science. 1994 Apr 22;264(5158):566-9 PMID: 7909170
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.22.4.1246-1252.2002
Published
2002-02-00
Pages
1246-52
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC134645
Subset
IM
Grants
NCI NIH HHS · R01CA77668 · United States
External Links
PubMed source DOI: 10.1128/MCB.22.4.1246-1252.2002 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2