Analysis of retrograde transport in motor neurons reveals common endocytic carriers for tetanus toxin and neurotrophin receptor p75NTR.
Axonal retrograde transport is essential for neuronal growth and survival. However, the nature and dynamics of the membrane compartments involved in this process are poorly characterized. To shed light on this pathway, we established an experimental system for the visualization and the quantitative study of retrograde transport in living motor neurons based on a fluorescent fragment of tetanus toxin (TeNT HC). Morphological and kinetic analysis of TeNT HC retrograde carriers reveals two major groups of organelles: round vesicles and fast tubular structures. TeNT HC carriers lack markers of the classical endocytic pathway and are not acidified during axonal transport. Importantly, TeNT HC and NGF share the same retrograde transport organelles, which are characterized by the presence of the neurotrophin receptor p75NTR. Our results provide the first direct visualization of retrograde transport in living motor neurons, and reveal a novel retrograde route that could be used both by physiological ligands (i.e., neurotrophins) and TeNT to enter the central nervous system.
27 references, click to expand
-
Lipid rafts act as specialized domains for tetanus toxin binding and internalization into neurons.Mol Biol Cell. 2001 Oct;12(10):2947-60 PMID: 11598183
-
Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular-transport pathway to the ER.Nat Cell Biol. 2001 May;3(5):473-83 PMID: 11331875
-
Retrograde transport of nerve growth factor (NGF) in motoneurons of developing rats: assessment of potential neurotrophic effects.Neuron. 1988 Jun;1(4):335-43 PMID: 2483324
-
Actin-dependent organelle movement in squid axoplasm.Nature. 1992 Apr 23;356(6371):722-5 PMID: 1570018
-
Axonal and dendritic endocytic pathways in cultured neurons.J Cell Biol. 1992 Oct;119(1):123-37 PMID: 1527164
-
Products of endocytosis and autophagy are retrieved from axons by regulated retrograde organelle transport.J Cell Biol. 1993 Apr;121(2):305-15 PMID: 7682217
-
Influences of neurotrophins on mammalian motoneurons in vivo.J Neurobiol. 1993 Dec;24(12):1555-77 PMID: 8301265
-
Axonal transport of mitochondria along microtubules and F-actin in living vertebrate neurons.J Cell Biol. 1995 Dec;131(5):1315-26 PMID: 8522592
-
Dynamic organization of endocytic pathways in axons of cultured sympathetic neurons.J Neurosci. 1996 Oct 1;16(19):6056-64 PMID: 8815888
-
Synaptic vesicle endocytosis mediates the entry of tetanus neurotoxin into hippocampal neurons.Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13310-5 PMID: 8917587
-
Retrograde transport and steady-state distribution of 125I-nerve growth factor in rat sympathetic neurons in compartmented cultures.J Neurosci. 1997 Feb 15;17(4):1282-90 PMID: 9006972
-
Visualization of the dynamics of synaptic vesicle and plasma membrane proteins in living axons.J Cell Biol. 1998 Feb 9;140(3):659-74 PMID: 9456325
-
Specific covalent labeling of recombinant protein molecules inside live cells.Science. 1998 Jul 10;281(5374):269-72 PMID: 9657724
-
Association of Rab25 and Rab11a with the apical recycling system of polarized Madin-Darby canine kidney cells.Mol Biol Cell. 1999 Jan;10(1):47-61 PMID: 9880326
-
Association of the p75 neurotrophin receptor with TRAF6.J Biol Chem. 1999 Jan 29;274(5):2597-600 PMID: 9915784
-
Functional characterisation of tetanus and botulinum neurotoxins binding domains.J Cell Sci. 1999 Aug;112 ( Pt 16):2715-24 PMID: 10413679
-
Dynamics of tubulovesicular recycling endosomes in hippocampal neurons.J Neurosci. 1999 Dec 1;19(23):10324-37 PMID: 10575030
-
Nerve growth factor signaling in caveolae-like domains at the plasma membrane.J Biol Chem. 1999 Dec 17;274(51):36707-14 PMID: 10593976
-
Neurotoxins affecting neuroexocytosis.Physiol Rev. 2000 Apr;80(2):717-66 PMID: 10747206
-
Axonal membrane proteins are transported in distinct carriers: a two-color video microscopy study in cultured hippocampal neurons.Mol Biol Cell. 2000 Apr;11(4):1213-24 PMID: 10749925
-
Tetanus toxin fragment C binds to a protein present in neuronal cell lines and motoneurons.J Neurochem. 2000 May;74(5):1941-50 PMID: 10800937
-
Microtubule-based transport systems in neurons: the roles of kinesins and dyneins.Annu Rev Neurosci. 2000;23:39-71 PMID: 10845058
-
Retrograde axonal transport of herpes simplex virus: evidence for a single mechanism and a role for tegument.Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):8146-50 PMID: 10884436
-
Involvement of cellular caveolae in bacterial entry into mast cells.Science. 2000 Aug 4;289(5480):785-8 PMID: 10926542
-
Signalling organelle for retrograde axonal transport of internalized neurotrophins from the nerve terminal.Immunol Cell Biol. 2000 Aug;78(4):430-5 PMID: 10947869
-
Endocytic traffic in polarized epithelial cells: role of the actin and microtubule cytoskeleton.Traffic. 2001 Mar;2(3):149-59 PMID: 11260520
-
Comparison between the retrograde axonal transport of nerve growth factor and tetanus toxin in motor, sensory and adrenergic neurons.Brain Res. 1975 Nov 28;99(1):1-16 PMID: 52914
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
