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PMID: 11805338 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 2 ·2002-01-22 ·Pages 966-71

Akerley BJ, Rubin EJ, Novick VL, Amaya K, Judson N, Mekalanos JJ

Abstract

A high-density transposon mutagenesis strategy was applied to the Haemophilus influenzae genome to identify genes required for growth or viability. This analysis detected putative essential roles for the products of 259 ORFs of unknown function. Comparisons between complete genomes defined a subset of these proteins in H. influenzae having homologs in Mycobacterium tuberculosis that are absent in Saccharomyces cerevisiae, a distribution pattern that favors their use in development of antimicrobial therapeutics. Three genes within this set are essential for viability in other bacteria. Interfacing the set of essential gene products in H. influenzae with the distribution of homologs in other microorganisms can detect components of unrecognized cellular pathways essential in diverse bacteria. This genome-scale phenotypic analysis identifies potential roles for a large set of genes of unknown function.

MeSH Terms
Base Sequence Chromosome Mapping Chromosomes, Bacterial/genetics Genes, Bacterial Genome, Bacterial Haemophilus influenzae/genetics,growth & development Mutagenesis, Insertional Open Reading Frames Phenotype Plasmids/genetics
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Akerley Brian J
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Rubin Eric J
Novick Veronica L
Amaya Kensey
Judson Nicholas
Mekalanos John J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.012602299
Published
2002-01-22
Pages
966-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC117414
Subset
IM
Grants
NIAID NIH HHS · R01 AI026289 · United States
NIAID NIH HHS · AI-48704 · United States
NIAID NIH HHS · R01 AI048704 · United States
NIAID NIH HHS · AI-26289 · United States
NIAID NIH HHS · AI-02137 · United States
External Links
PubMed source DOI: 10.1073/pnas.012602299 PMC Full Text View journal details
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