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PMID: 11799200 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differential activation of influenza A virus endonuclease activity is dependent on multiple sequence differences between the virion RNA and cRNA promoters.

Journal of virology ·Vol. 76 ·No. 4 ·2002-02-00 ·Pages 2019-23

Leahy MB, Zecchin G, Brownlee GG

Abstract

Influenza virus endonuclease activity was studied in vitro with model virion RNA (vRNA) and cRNA molecules. We show that endonuclease activity can be partially rescued by transplanting vRNA-like promoter features into the model cRNA promoter. This study defines three distinctive features within the vRNA promoter--absent in the cRNA promoter--that are required for endonuclease cleavage.

MeSH Terms
Base Sequence Endonucleases/metabolism Enzyme Activation Gene Expression Regulation, Viral HeLa Cells Humans Influenza A virus/enzymology,genetics Influenza, Human/virology Molecular Sequence Data Mutation Promoter Regions, Genetic/genetics RNA, Complementary/genetics RNA, Viral/genetics Sequence Analysis, DNA Virion/genetics
Chemicals
RNA, Complementary RNA, Viral Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Leahy Michael B
Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
Zecchin Giuseppe
Brownlee George G
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16 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.76.4.2019-2023.2002
Published
2002-02-00
Pages
2019-23
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC135883
Subset
IM
External Links
PubMed source DOI: 10.1128/jvi.76.4.2019-2023.2002 PMC Full Text View journal details
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