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PMID: 11780634 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Small bristles, the Drosophila ortholog of NXF-1, is essential for mRNA export throughout development.

RNA (New York, N.Y.) ·Vol. 7 ·No. 12 ·2001-12-00 ·Pages 1781-92

Wilkie GS, Zimyanin V, Kirby R, Korey C, Francis-Lang H, Van Vactor D, Davis I

Abstract

We identified a temperature-sensitive allele of small bristles (sbr), the Drosophila ortholog of human TAP/NXF-1 and yeast Mex67, in a screen for mutants defective in mRNA export. We show that sbr is essential for the nuclear export of all mRNAs tested in a wide range of tissues and times in development. High resolution and sensitive in situ hybridization detect the rapid accumulation of individual mRNA species in sbr mutant nuclei in particles that are distinct from nascent transcript foci and resemble wild-type export intermediates. The particles become more numerous and intense with increasing time at the restrictive temperature and are exported very rapidly after shifting back to the permissive temperature. The mRNA export block is not due indirectly to a defect in splicing, nuclear protein import, or aberrant nuclear ultrastructure, suggesting that in sbr mutants, mRNA is competent for export but fails to dock or translocate through NPCs. We conclude that NXF-1 is an essential ubiquitous export factor for all mRNAs throughout development in higher eukaryotes.

MeSH Terms
Active Transport, Cell Nucleus Amino Acid Sequence Animals Blastoderm/metabolism Cell Nucleus/metabolism,ultrastructure Drosophila/embryology,genetics Drosophila Proteins/genetics,metabolism Molecular Sequence Data Mutation Nuclear Proteins/genetics Nucleocytoplasmic Transport Proteins RNA, Messenger/metabolism RNA-Binding Proteins/genetics,metabolism
Chemicals
Drosophila Proteins NXF1 protein, human Nuclear Proteins Nucleocytoplasmic Transport Proteins RNA, Messenger RNA-Binding Proteins SBR protein, Drosophila
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wilkie G S
Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, Scotland, United Kingdom.
Zimyanin V
Kirby R
Korey C
Francis-Lang H
Van Vactor D
Davis I
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
Published
2001-12-00
Pages
1781-92
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1370217
Subset
IM
Grants
NIGMS NIH HHS · GM46409 · United States
NINDS NIH HHS · NS35909 · United States
NINDS NIH HHS · NS40043 · United States
Databases
GENBANK
AJ251947
External Links
PubMed source PMC Full Text View journal details
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