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PMID: 1176628 Published · ppublish English Comparative Study Journal Article

Improved auxanographic method for yeast assimilations: a comparison with other approaches.

Journal of clinical microbiology ·Vol. 2 ·No. 3 ·1975-09-00 ·Pages 206-17

Land GA, Vinton EC, Adcock GB, Hopkins JM

Abstract

An improved pour-plate auxanographic method has been developed for determining the assimilation of 14 different carbohydrates by medically important yeasts. Reduction of a dye incorporated into the agar has been correlated with the growth and carbohydrate assimilation of the yeasts, allowing the speciation of many yeasts within 24 to 48 h. This technique has been found to compare more than favorably with existing yeast assimilation techniques, in terms of rapid identification, total cost, and technician time in preparing and inoculating the plates. The dye pour-plate auxanographic technique provides an easier-to-interpret, rapid, and reproducible method of mycological identification for small clinical laboratories.

MeSH Terms
Agar Carbohydrate Metabolism Classification/methods Evaluation Studies as Topic Indicators and Reagents Species Specificity Yeasts/classification,growth & development,metabolism
Chemicals
Indicators and Reagents Agar
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Land G A
Vinton E C
Adcock G B
Hopkins J M
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25 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.2.3.206-217.1975
Published
1975-09-00
Pages
206-17
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC274172
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.2.3.206-217.1975 PMC Full Text View journal details
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