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PMID: 11752381 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tomato SP-interacting proteins define a conserved signaling system that regulates shoot architecture and flowering.

The Plant cell ·Vol. 13 ·No. 12 ·2001-12-00 ·Pages 2687-702

Pnueli L, Gutfinger T, Hareven D, Ben-Naim O, Ron N, Adir N, Lifschitz E

Abstract

Divergent architecture of shoot models in flowering plants reflects the pattern of production of vegetative and reproductive organs from the apical meristem. The SELF-PRUNING (SP) gene of tomato is a member of a novel CETS family of regulatory genes (CEN, TFL1, and FT) that controls this process. We have identified and describe here several proteins that interact with SP (SIPs) and with its homologs from other species: a NIMA-like kinase (SPAK), a bZIP factor, a novel 10-kD protein, and 14-3-3 isoforms. SPAK, by analogy with Raf1, has two potential binding sites for 14-3-3 proteins, one of which is shared with SP. Surprisingly, overexpression of 14-3-3 proteins partially ameliorates the effect of the sp mutation. Analysis of the binding potential of chosen mutant SP variants, in relation to conformational features known to be conserved in this new family of regulatory proteins, suggests that associations with other proteins are required for the biological function of SP and that ligand binding and protein-protein association domains of SP may be separated. We suggest that CETS genes encode a family of modulator proteins with the potential to interact with a variety of signaling proteins in a manner analogous to that of 14-3-3 proteins.

MeSH Terms
14-3-3 Proteins Amino Acid Sequence Arabidopsis/genetics,metabolism Basic-Leucine Zipper Transcription Factors Binding Sites/genetics Gene Expression Regulation, Plant Lycopersicon esculentum/genetics,metabolism Molecular Sequence Data Mutagenesis Mutation Phenotype Plant Proteins/genetics,metabolism Plant Stems/genetics,metabolism Plants, Genetically Modified Protein Binding Protein Serine-Threonine Kinases/genetics,metabolism Saccharomyces cerevisiae Proteins Scrophulariaceae/genetics,metabolism Signal Transduction Species Specificity Trans-Activators/genetics,metabolism Tyrosine 3-Monooxygenase/genetics,metabolism
Chemicals
14-3-3 Proteins Basic-Leucine Zipper Transcription Factors Plant Proteins SIP4 protein, S cerevisiae SP protein, Lycopersicon esculentum Saccharomyces cerevisiae Proteins Trans-Activators Tyrosine 3-Monooxygenase Protein Serine-Threonine Kinases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Pnueli L
Department of Biology, Science and Technology, Technion, Israel Institute of Technology 32000, Haifa, Israel.
Gutfinger T
Hareven D
Ben-Naim O
Ron N
Adir N
Lifschitz E
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.010293
Published
2001-12-00
Pages
2687-702
Language
English
Region
England
NLM ID
9208688
PMCID
PMC139482
Subset
IM
Databases
GENBANK
AF079103, AF079104, AF079450, AF079451, AF175963
External Links
PubMed source DOI: 10.1105/tpc.010293 PMC Full Text View journal details
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