Abstract
We have developed a system for producing murine leukemia virus (MLV) pseudotyped with human hepatitis B virus (HBV) large (L) and small (S) surface antigens (HBsAg) for targeting primary human hepatocytes. Using the MLV(HBV) pseudotype virus containing a beta-galactosidase reporter gene, we demonstrated that this pseudotype virus exhibits strict tropism for primary human hepatocytes, similar to the natural target cell specificity of HBV. It does not infect any of the established tissue culture cell lines, including human hepatoma cell lines (HepG2 and Huh-7), or rat primary hepatocytes. The infectivity of MLV(HBV) for human hepatocytes was inhibited by anti-HBs antibody. The L form of HBsAg was both necessary and sufficient for virus infectivity, but the presence of both L and S forms enhanced the surface expression of HBsAg and thus increased virus production. The middle form of HBsAg was not necessary. This pseudotype virus bypasses the requirement for the liver-specific transcription factors for HBV replication, enabling direct study of HBV tissue tropism conferred by the viral envelope proteins. This virus also offers a potential liver-specific targeting system for gene therapy.
MeSH Terms
Animals
Cells, Cultured
Genetic Vectors/genetics
Hepatitis B Surface Antigens/genetics,immunology
Hepatitis B virus/genetics,immunology
Hepatocytes/metabolism,virology
Humans
Mice/virology
Rats
Retroviridae/chemistry,genetics,physiology
Sensitivity and Specificity
Species Specificity
Transcription Factors/metabolism
Tumor Cells, Cultured
Virus Replication
Chemicals
Hepatitis B Surface Antigens
Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sung Vicky M H
Department of Molecular Microbiology and Immunology, Howard Hughes Medical Institute, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA.
Lai Michael M C
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