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PMID: 11742984 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A PEST-like element in FREQUENCY determines the length of the circadian period in Neurospora crassa.

The EMBO journal ·Vol. 20 ·No. 24 ·2001-12-17 ·Pages 7074-84

Görl M, Merrow M, Huttner B, Johnson J, Roenneberg T, Brunner M

Abstract

FREQUENCY (FRQ) is a crucial element of the circadian clock in Neurospora crassa. In the course of a circadian day FRQ is successively phosphorylated and degraded. Here we report that two PEST-like elements in FRQ, PEST-1 and PEST-2, are phosphorylated in vitro by recombinant CK-1a and CK-1b, two newly identified Neurospora homologs of casein kinase 1 epsilon. CK-1a is localized in the cytosol and the nuclei of Neurospora and it is in a complex with FRQ in vivo. Deletion of PEST-1 results in hypophosphorylation of FRQ and causes significantly increased protein stability. A strain harboring the mutant frq Delta PEST-1 gene shows no rhythmic conidiation. Despite the lack of overt rhythmicity, frq Delta PEST-1 RNA and FRQ Delta PEST-1 protein are rhythmically expressed and oscillate in constant darkness with a circadian period of 28 h. Thus, by deletion of PEST-1 the circadian period is lengthened and overt rhythmicity is dissociated from molecular oscillations of clock components.

MeSH Terms
Amino Acid Sequence Base Sequence Casein Kinases Circadian Rhythm DNA Primers Fungal Proteins/chemistry,physiology Molecular Sequence Data Neurospora crassa/physiology Phosphorylation Protein Kinases/chemistry,metabolism Recombinant Proteins/chemistry,metabolism
Chemicals
DNA Primers FRQ protein, Neurospora crassa Fungal Proteins Recombinant Proteins Protein Kinases Casein Kinases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Görl M
Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg Germany.
Merrow M
Huttner B
Johnson J
Roenneberg T
Brunner M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.24.7074
Published
2001-12-17
Pages
7074-84
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125781
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/20.24.7074 PMC Full Text View journal details
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