PIP3, PIP2, and cell movement--similar messages, different meanings?

Developmental cell ·Vol. 1 ·No. 6 ·2001-12-00 ·Pages 743-7

Insall RH, Weiner OD

Abstract

The inositol lipids PI(4,5)P(2) and PI(3,4,5)P(3) are important regulators of actin polymerization, but their different temporal and spatial dynamics suggest that they perform separate roles. PI(3,4,5)P(3) seems to act as an instructive second messenger, inducing local actin polymerization. PI(4,5)P(2) appears to be present at too high a concentration and homogeneous a distribution to fulfil a similar role. Instead, we suggest that PI(4,5)P(2) acts permissively, restricting new actin polymerization to the region of the plasma membrane.

MeSH Terms

Actins/metabolism Cell Membrane/metabolism Cell Movement/physiology Cytoskeleton/metabolism Neutrophils/metabolism Phosphatidylinositol 4,5-Diphosphate/metabolism Phosphatidylinositols/metabolism Polymers/chemistry Proteins/metabolism Second Messenger Systems/physiology Signal Transduction/physiology Wiskott-Aldrich Syndrome Protein

Chemicals

Actins Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositols Polymers Proteins Wiskott-Aldrich Syndrome Protein phosphoinositide-3,4,5-triphosphate

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Insall R H

School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom. r.h.insall@bham.ac.uk

Weiner O D

References (24)

24 references, click to expand

G protein signaling events are activated at the leading edge of chemotactic cells.
Cell. 1998 Oct 2;95(1):81-91 PMID: 9778249
Coupling of Ras and Rac guanosine triphosphatases through the Ras exchanger Sos.
Science. 1998 Jan 23;279(5350):560-3 PMID: 9438849
Role of substrates and products of PI 3-kinase in regulating activation of Rac-related guanosine triphosphatases by Vav.
Science. 1998 Jan 23;279(5350):558-60 PMID: 9438848
The phosphatidylinositol 3-kinase inhibitor wortmannin markedly reduces chemotactic peptide-induced locomotion and increases in cytoskeletal actin in human neutrophils.
Eur J Pharmacol. 1997 Sep 17;335(1):43-52 PMID: 9371545
The lipid products of phosphoinositide 3-kinase increase cell motility through protein kinase C.
J Biol Chem. 1997 Mar 7;272(10):6465-70 PMID: 9045671
Thrombin receptor ligation and activated Rac uncap actin filament barbed ends through phosphoinositide synthesis in permeabilized human platelets.
Cell. 1995 Aug 25;82(4):643-53 PMID: 7664343
Phosphoinositide 3-kinase inhibition spares actin assembly in activating platelets but reverses platelet aggregation.
J Biol Chem. 1995 May 12;270(19):11358-66 PMID: 7744773
Activation of phosphoinositide 3-kinase is required for PDGF-stimulated membrane ruffling.
Curr Biol. 1994 May 1;4(5):385-93 PMID: 7922352
Agonist-stimulated synthesis of phosphatidylinositol(3,4,5)-trisphosphate: a new intracellular signalling system?
Biochim Biophys Acta. 1993 Oct 7;1179(1):27-75 PMID: 8399352
Pathway of phosphatidylinositol(3,4,5)-trisphosphate synthesis in activated neutrophils.
Nature. 1991 May 2;351(6321):33-9 PMID: 1851250
Polarization of chemoattractant receptor signaling during neutrophil chemotaxis.
Science. 2000 Feb 11;287(5455):1037-40 PMID: 10669415
How WASP-family proteins and the Arp2/3 complex convert intracellular signals into cytoskeletal structures.
Curr Opin Cell Biol. 2000 Feb;12(1):91-6 PMID: 10679362
Chemoattractant-mediated transient activation and membrane localization of Akt/PKB is required for efficient chemotaxis to cAMP in Dictyostelium.
EMBO J. 1999 Apr 15;18(8):2092-105 PMID: 10205164
Characterization of rac and cdc42 activation in chemoattractant-stimulated human neutrophils using a novel assay for active GTPases.
J Biol Chem. 1999 May 7;274(19):13198-204 PMID: 10224076
alphaPIX nucleotide exchange factor is activated by interaction with phosphatidylinositol 3-kinase.
Oncogene. 1999 Oct 7;18(41):5680-90 PMID: 10523848
PDGF stimulates an increase in GTP-Rac via activation of phosphoinositide 3-kinase.
Curr Biol. 1995 Apr 1;5(4):393-403 PMID: 7627555
A membrane-permeant ester of phosphatidylinositol 3,4, 5-trisphosphate (PIP(3)) is an activator of human neutrophil migration.
FEBS Lett. 2000 May 12;473(2):217-21 PMID: 10812078
An actin-nucleating activity in polymorphonuclear leukocytes is modulated by chemotactic peptides.
J Cell Biol. 1986 Dec;103(6 Pt 2):2707-14 PMID: 3793753
The kinetics of chemotactic peptide-induced change in F-actin content, F-actin distribution, and the shape of neutrophils.
J Cell Biol. 1985 Sep;101(3):1078-85 PMID: 4040915
Role of phosphatidylinositol 3' kinase and a downstream pleckstrin homology domain-containing protein in controlling chemotaxis in dictyostelium.
J Cell Biol. 2001 May 14;153(4):795-810 PMID: 11352940
Spatial sensing in fibroblasts mediated by 3' phosphoinositides.
J Cell Biol. 2000 Dec 11;151(6):1269-80 PMID: 11121441
Signal transduction. N-WASP regulation--the sting in the tail.
Science. 2000 Oct 27;290(5492):725-6 PMID: 11184204
Leukocytes navigate by compass: roles of PI3Kgamma and its lipid products.
Trends Cell Biol. 2000 Nov;10(11):466-73 PMID: 11050418
Two pathways through Cdc42 couple the N-formyl receptor to actin nucleation in permeabilized human neutrophils.
J Cell Biol. 2000 Aug 21;150(4):785-96 PMID: 10953003

Article Info

Journal

Developmental cell

Abbr.

Dev Cell

ISSN

1534-5807

DOI

10.1016/s1534-5807(01)00086-7

r.h.insall@bham.ac.uk

Published

2001-12-00

Pages

743-7

Language

English

Region

United States

NLM ID

101120028

PMCID

PMC2819114

Subset

Grants

NIGMS NIH HHS · R01 GM027800-27 · United States

External Links

PubMed source DOI: 10.1016/s1534-5807(01)00086-7 PMC Full Text View journal details

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PIP3, PIP2, and cell movement--similar messages, different meanings?

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