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PMID: 11722934 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence analysis of a 101-kilobase plasmid required for agar degradation by a Microscilla isolate.

Applied and environmental microbiology ·Vol. 67 ·No. 12 ·2001-12-00 ·Pages 5771-9

Zhong Z, Toukdarian A, Helinski D, Knauf V, Sykes S, Wilkinson JE, O'Bryne C, Shea T, DeLoughery C, Caspi R

Abstract

An agar-degrading marine bacterium identified as a Microscilla species was isolated from coastal California marine sediment. This organism harbored a single 101-kb circular DNA plasmid designated pSD15. The complete nucleotide sequence of pSD15 was obtained, and sequence analysis indicated a number of genes putatively encoding a variety of enzymes involved in polysaccharide utilization. The most striking feature was the occurrence of five putative agarase genes. Loss of the plasmid, which occurred at a surprisingly high frequency, was associated with loss of agarase activity, supporting the sequence analysis results.

MeSH Terms
Agar/metabolism Bacteria/enzymology,genetics,growth & development Bacterial Proteins/genetics,metabolism Biodegradation, Environmental DNA Replication Geologic Sediments/microbiology Glycoside Hydrolases/genetics,metabolism Molecular Sequence Data Plasmids/genetics Polysaccharides/metabolism Seawater/microbiology Sequence Analysis, DNA
Chemicals
Bacterial Proteins Polysaccharides Agar Glycoside Hydrolases agarase
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Zhong Z
Division of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093-0322, USA.
Toukdarian A
Helinski D
Knauf V
Sykes S
Wilkinson J E
O'Bryne C
Shea T
DeLoughery C
Caspi R
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.67.12.5771-5779.2001
Published
2001-12-00
Pages
5771-9
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC93371
Subset
IM
Databases
GENBANK
AF339846
External Links
PubMed source DOI: 10.1128/AEM.67.12.5771-5779.2001 PMC Full Text View journal details
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