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PMID: 11707418 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps.

The EMBO journal ·Vol. 20 ·No. 22 ·2001-11-15 ·Pages 6475-84

Saveanu C, Bienvenu D, Namane A, Gleizes PE, Gas N, Jacquier A, Fromont-Racine M

Abstract

Eukaryotic ribosome maturation depends on a set of well ordered processing steps. Here we describe the functional characterization of yeast Nog2p (Ynr053cp), a highly conserved nuclear protein. Nog2p contains a putative GTP-binding site, which is essential in vivo. Kinetic and steady-state measurements of the levels of pre-rRNAs in Nog2p-depleted cells showed a defect in 5.8S and 25S maturation and a concomitant increase in the levels of both 27SB(S) and 7S(S) precursors. We found Nog2p physically associated with large pre-60S complexes highly enriched in the 27SB and 7S rRNA precursors. These complexes contained, besides a subset of ribosomal proteins, at least two additional factors, Nog1p, another putative GTP-binding protein, and Rlp24p (Ylr009wp), which belongs to the Rpl24e family of archaeal and eukaryotic ribosomal proteins. In the absence of Nog2p, the pre-60S ribosomal complexes left the nucleolus, but were retained in the nucleoplasm. These results suggest that transient, possibly GTP-dependent association of Nog2p with the pre-ribosomes might trigger late rRNA maturation steps in ribosomal large subunit biogenesis.

MeSH Terms
Active Transport, Cell Nucleus Alternative Splicing Amino Acid Sequence Binding Sites Blotting, Northern Cell Nucleolus/metabolism Cell Nucleus/metabolism Cytoplasm/metabolism DNA, Complementary/metabolism GTP Phosphohydrolases/chemistry,genetics,metabolism GTP-Binding Proteins/metabolism Genotype Glucose/metabolism Green Fluorescent Proteins Humans In Situ Hybridization Kinetics Luminescent Proteins/metabolism Mass Spectrometry Microscopy, Electron Microscopy, Fluorescence Models, Genetic Molecular Sequence Data Plasmids/metabolism Polyribosomes/metabolism Promoter Regions, Genetic Protein Binding RNA/metabolism RNA, Messenger/metabolism RNA, Ribosomal/metabolism Ribosomes/chemistry,metabolism Sequence Homology, Amino Acid Time Factors
Chemicals
DNA, Complementary Luminescent Proteins RNA, Messenger RNA, Ribosomal Green Fluorescent Proteins RNA GTP Phosphohydrolases GTP-Binding Proteins Glucose
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Saveanu C
Génétique des Interactions Macromoléculaires, Institut Pasteur (CNRS-URA2171), 25-28 rue du Dr Roux, 75724 Paris Cedex 15, France.
Bienvenu D
Namane A
Gleizes P E
Gas N
Jacquier A
Fromont-Racine M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.22.6475
Published
2001-11-15
Pages
6475-84
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125736
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/20.22.6475 PMC Full Text View journal details
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