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PMID: 11696441 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The transcription factor PU.1, necessary for B-cell development is expressed in lymphocyte predominance, but not classical Hodgkin's disease.

The American journal of pathology ·Vol. 159 ·No. 5 ·2001-11-00 ·Pages 1807-14

Torlakovic E, Tierens A, Dang HD, Delabie J

Abstract

Hodgkin's disease (HD) is a lymphoproliferative disease of predominantly B-cell origin. However, the reasons for the incomplete development of the B-cell phenotype and lack of immunoglobulin expression in classical HD (cHD) have not been fully explained. We examined the expression of PU.1 in HD, an Ets-family transcription factor, which regulates the expression of immunoglobulin and other genes that are important for B-cell development. Immunohistochemistry for PU.1 was performed on 35 cases of cHD and 15 cases of lymphocyte predominance HD as well as 67 non-Hodgkin's lymphomas (NHL). Expression of PU.1 was studied by Western blotting in four cHD-derived cell lines and in five NHL cell lines. We also studied the expression of two additional B-cell transcription factors, B-cell-specific activator protein and Oct-2. Our results show a striking lack of PU.1 expression by neoplastic cells in cHD but not in lymphocyte predominance HD. Our study also confirmed that B-cell-specific activator protein but not Oct-2 is not expressed by cHD. Western blotting showed no PU.1 protein expression in the cHD-derived cell lines, with the exception of one cell line of putative monocyte/histiocyte origin. The lack of PU.1 protein expression in cHD likely contributes to the lack of immunoglobulin expression and incomplete B-cell phenotype characteristic of the Reed-Sternberg cells in cHD.

MeSH Terms
Blotting, Western DNA-Binding Proteins/metabolism Hodgkin Disease/classification,metabolism,pathology Humans Immunohistochemistry Lymph Nodes/metabolism,pathology Lymphocytes/metabolism,pathology Lymphoma, Non-Hodgkin/metabolism Octamer Transcription Factor-2 Phenotype Proto-Oncogene Proteins/physiology Trans-Activators/physiology Transcription Factors/metabolism Tumor Cells, Cultured
Chemicals
DNA-Binding Proteins Octamer Transcription Factor-2 POU2F2 protein, human Proto-Oncogene Proteins Trans-Activators Transcription Factors proto-oncogene protein Spi-1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Torlakovic E
Department of Pathology, The Norwegian Radium Hospital, Oslo, Norway. emina.torlakovic@labmed.uio.no
Tierens A
Dang H D
Delabie J
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
DOI
10.1016/S0002-9440(10)63027-1
Email
emina.torlakovic@labmed.uio.no
Published
2001-11-00
Pages
1807-14
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1867045
Subset
IM
External Links
PubMed source DOI: 10.1016/S0002-9440(10)63027-1 PMC Full Text View journal details
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