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PMID: 11691832 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Whi3 binds the mRNA of the G1 cyclin CLN3 to modulate cell fate in budding yeast.

Genes & development ·Vol. 15 ·No. 21 ·2001-11-01 ·Pages 2803-8

Garí E, Volpe T, Wang H, Gallego C, Futcher B, Aldea M

Abstract

Eukaryotic cells commit in G1 to a new mitotic cycle or to diverse differentiation processes. Here we show that Whi3 is a negative regulator of Cln3, a G1 cyclin that promotes transcription of many genes to trigger the G1/S transition in budding yeast. Whi3 contains an RNA-recognition motif that specifically binds the CLN3 mRNA, with no obvious effects on Cln3 levels, and localizes the CLN3 mRNA into discrete cytoplasmic foci. This is the first indication that G1 events may be regulated by locally restricting the synthesis of a cyclin. Moreover, Whi3 is also required for restraining Cln3 function in meiosis, filamentation, and mating, thus playing a key role in cell fate determination in budding yeast.

MeSH Terms
Amino Acid Motifs Blotting, Northern Cell Lineage Cyclin G Cyclins/chemistry,metabolism Cytoplasm/metabolism Fungal Proteins/chemistry,metabolism In Situ Hybridization, Fluorescence Meiosis Microscopy, Fluorescence Models, Biological Plasmids/metabolism Protein Binding RNA/metabolism RNA, Messenger/metabolism RNA-Binding Proteins/metabolism,physiology Saccharomyces cerevisiae Proteins Saccharomycetales/physiology Time Factors
Chemicals
CLN3 protein, S cerevisiae Cyclin G Cyclins Fungal Proteins RNA, Messenger RNA-Binding Proteins Saccharomyces cerevisiae Proteins Whi3 protein, S cerevisiae RNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Garí E
Departament de Ciències Mèdiques Bàsiques, Universitat de Lleida, 25198 Lleida, Catalunya, Spain.
Volpe T
Wang H
Gallego C
Futcher B
Aldea M
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.203501
Published
2001-11-01
Pages
2803-8
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC312816
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.203501 PMC Full Text View journal details
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