3H-uridine incorporation in the puff 93D and in chromocentric heterochromatin of heat shocked salivary glands of Drosophila melanogaster.

Chromosoma ·Vol. 74 ·No. 1 ·1979-09-01 ·Pages 75-82

Mukherjee T, Lakhotia SC

Abstract

The autoradiographic patterns of 3H-uridine labelling of the major temperature shock puff sites and the chromocentric (beta-)heterochromatin in heat shocked (37 degrees C) salivary glands of Drosophila melanogaster have been studied. It is seen that in response to the heat shock treatment, four of the major temperature shock puffs (63BC, 87A, 87C and 95D) show a correlated level of 3H-uridine incorporation in a given nucleus. However, although the mean grain density on 93D puff is maximum in the heat shocked preparations, in individual nuclei this puff is labelled to a widely varying level and this variation in its labelling is statistically not correlated to the labelling of the other four temperature shock puffs in a nucleus. The chromocentric beta-heterochromatin, which has been shown in several earlier studies to hybridize with temperature shock RNA fractions, is seen to be totally inactive in transcription after the heat shock.

MeSH Terms

Animals Autoradiography Drosophila melanogaster/genetics,metabolism Heterochromatin/metabolism Hot Temperature RNA/biosynthesis Salivary Glands/metabolism Transcription, Genetic Tritium Uridine/metabolism

Chemicals

Heterochromatin Tritium RNA Uridine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Mukherjee T

Lakhotia S C

References (12)

12 references, click to expand

Messenger RNA in heat-shocked Drosophila cells.
J Mol Biol. 1977 Feb 5;109(4):559-87 PMID: 403287
Localization of RNA from heat-induced polysomes at puff sites in Drosophila melanogaster.
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1117-21 PMID: 805422
Induction of the Drosophila heat shock response in isolated polytene nuclei.
Cell. 1978 May;14(1):191-201 PMID: 667933
Analysis of drosophila mRNA by in situ hybridization: sequences transcribed in normal and heat shocked cultured cells.
Cell. 1975 Apr;4(4):395-404 PMID: 1122559
Replication in Drosophila chromosomes. I. Replication of intranucleolar DNA in polytene cells of D. nasuta.
J Cell Sci. 1979 Apr;36:185-97 PMID: 457805
The effect of heat shock on RNA synthesis in Drosophila tissues.
Cell. 1976 May;8(1):43-50 PMID: 954091
EM autoradiographic studies on polytene nuclei of Drosophila melanogaster. II. Organization and transcriptive activity of the chromocentre.
Exp Cell Res. 1974 Jun;86(2):253-63 PMID: 4210378
Deletions of two heat-activated loci in Drosophila melanogaster and their effects on heat-induced protein synthesis.
Cell. 1977 Nov;12(3):643-52 PMID: 411575
The effects of protein synthesis-inhibiting antibiotics on the puffing pattern of Drosophila salivary gland chromosomes in vitro.
Cell Differ. 1973 May;2(2):97-106 PMID: 4209554
Patterns of puffing activity in the salivary gland chromosomes of Drosophila. V. Responses to environmental treatments.
Chromosoma. 1970;31(3):356-76 PMID: 5489366
Sequence organization and transcription at two heat shock loci in Drosophila.
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5613-7 PMID: 103099
EXPERIMENTAL ACTIVATION OF SPECIFIC LOCI IN POLYTENE CHROMOSOMES OF DROSOPHILA.
Exp Cell Res. 1964 Sep;35:601-7 PMID: 14208747

Article Info

Journal

Chromosoma

Abbr.

Chromosoma

ISSN

0009-5915

DOI

10.1007/BF00344484

Published

1979-09-01

Pages

75-82

Language

English

Region

Austria

NLM ID

2985138R

Subset

External Links

PubMed source DOI: 10.1007/BF00344484 View journal details

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3H-uridine incorporation in the puff 93D and in chromocentric heterochromatin of heat shocked salivary glands of Drosophila melanogaster.

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