Determining confidence intervals when measuring genetic diversity and the discriminatory abilities of typing methods for microorganisms.

Journal of clinical microbiology ·Vol. 39 ·No. 11 ·2001-11-00 ·Pages 4190-2

Grundmann H, Hori S, Tanner G

Abstract

We describe here a method for determining confidence intervals for a commonly used index of diversity. This approach facilitates the comparison of the genetic population structure of microorganisms isolated from different environments and improves the objective assessment of the discriminatory power of typing techniques.

MeSH Terms

Bacterial Typing Techniques Carrier State/microbiology Confidence Intervals Deoxyribonucleases, Type II Site-Specific Electrophoresis, Gel, Pulsed-Field Genetic Variation Hospitalization Humans Random Amplified Polymorphic DNA Technique Staphylococcal Infections/microbiology Staphylococcus aureus/classification,genetics

Chemicals

CCCGGG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Grundmann H

Division of Microbiology and Infectious Diseases, University Hospital Nottingham, University of Nottingham, Nottingham NG7 2UH, United Kingdom. Hajo.Grundmann@Nottingham.ac.uk

Hori S

Tanner G

References (5)

5 references, click to expand

Consensus guidelines for appropriate use and evaluation of microbial epidemiologic typing systems.
Clin Microbiol Infect. 1996 Aug;2(1):2-11 PMID: 11866804
Multicenter study using standardized protocols and reagents for evaluation of reproducibility of PCR-based fingerprinting of Acinetobacter spp.
J Clin Microbiol. 1997 Dec;35(12):3071-7 PMID: 9399496
Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.
J Clin Microbiol. 1995 Sep;33(9):2233-9 PMID: 7494007
Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity.
J Clin Microbiol. 1988 Nov;26(11):2465-6 PMID: 3069867
Analysis of an outbreak of non-phage-typeable methicillin-resistant Staphylococcus aureus by using a randomly amplified polymorphic DNA assay.
J Clin Microbiol. 1997 Dec;35(12):3092-7 PMID: 9399500

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/JCM.39.11.4190-4192.2001

hajo.grundmann@nottingham.ac.uk

Published

2001-11-00

Pages

4190-2

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC88515

Subset

External Links

PubMed source DOI: 10.1128/JCM.39.11.4190-4192.2001 PMC Full Text View journal details

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Determining confidence intervals when measuring genetic diversity and the discriminatory abilities of typing methods for microorganisms.

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