Chick embryo cells infected with Japanese encephalitis virus were separated into smooth and rough membrane fractions, as judged by electron microscopy. Polyacrylamide gel electrophoretic analysis of the membrane fractions showed them to contain all of the virus specific polypeptides; however, the relative proportions of the polypeptides varied among smooth and rough membranes. The relationship of these observations to current concepts of group B arbovirus morphogenesis is discussed.
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