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PMID: 1167915 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Differential accumulation of virus-specific RNA during the cell cycle of adenovirus-transformed rat embyro cells.

Journal of virology ·Vol. 15 ·No. 4 ·1975-04-00 ·Pages 806-11

Hoffmann PR, Darnell JE

Abstract

In adenovirus type 2-transformed rat embryo cells there is a threefold greater incorporation of [3-H]uridine into virus-specific RNA early in S phase than in late S or G2. This heightened accumulation of labeled RNA is true for both nuclear and cytoplasmic virus-specific labeling. Inhibition of DNA synthesis decreases the virus-specific RNA labeling, whereas reversal of inhibition again allows the elevated level of virus-specific RNA labeling.

MeSH Terms
Adenoviridae Animals Cell Division Cell Nucleus/metabolism Cell Transformation, Neoplastic Cells, Cultured/drug effects,metabolism Cytoplasm/metabolism Hydroxyurea/pharmacology RNA, Viral/biosynthesis Rats/embryology Tritium Uridine
Chemicals
RNA, Viral Tritium Uridine Hydroxyurea
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hoffmann P R
Darnell J E
References (24)
24 references, click to expand
  1. Production of viral mRNA in adenovirus- transformed cells by the post- transcriptional processing of heterogeneous nuclear RNA containing viral and cell sequences.
    J Virol. 1973 Jun;11(6):953-60 PMID: 4736534
  2. The relationship between rates of ( 3 H)uridine and ( 3 H)adenine incorporation into RNA and the measured rates of RNA synthesis during the cell cycle.
    Biochim Biophys Acta. 1972 Sep 29;281(1):45-54 PMID: 4673465
  3. SEPARATION OF THE RNA MESSAGE TRANSCRIBED IN RESPONSE TO A SPECIFIC INDUCER.
    Proc Natl Acad Sci U S A. 1963 May;49(5):729-36 PMID: 16591095
  4. PYRIMIDINE METABOLISM IN TISSUE CULTURE CELLS DERIVED FROM RAT HEPATOMAS. 3. RELATIONSHIP OF THYMIDINE TO THE METABOLISM OF OTHER PYRIMIDINE NUCLEOSIDES IN SUSPENSION CULTURES DERIVED FROM THE NOVIKOFF HEPATOMA.
    Cancer Res. 1965 May;25:517-25 PMID: 14302036
  5. Deoxyriboside control and synchronization of mitosis.
    Nature. 1962 May 19;194:682-3 PMID: 14008663
  6. SPECIFIC COMPLEMENT-FIXING ANTIGENS IN POLYOMA TUMORS AND TRANSFORMED CELLS.
    Virology. 1965 Jan;25:55-61 PMID: 14277085
  7. QUANTITY PRODUCTION OF SYNCHRONIZED MAMMALIAN CELLS IN SUSPENSION CULTURE.
    Nature. 1964 Aug 8;203:642-3 PMID: 14250978
  8. Synthesis of RNA and protein during mitosis in mammalian tissue culture cells.
    Exp Cell Res. 1962 Mar;26:260-8 PMID: 14488623
  9. Nucleic acid synthesis in relation to the cell division cycle.
    Ann N Y Acad Sci. 1960 Oct 7;90:409-21 PMID: 13775619
  10. Localization and kinetics of formation of nuclear heterodisperse RNA, cytoplasmic heterodisperse RNA and polyribosome-associated messenger RNA in HeLa cells.
    J Mol Biol. 1968 May 28;34(1):49-60 PMID: 4106524
  11. The integrated state of viral DNA in SV40-transformed cells.
    Proc Natl Acad Sci U S A. 1968 Aug;60(4):1288-95 PMID: 4299943
  12. Temperature-dependent properties of cells transformed by a thermosensitive mutant of polyoma virus.
    Proc Natl Acad Sci U S A. 1970 Dec;67(4):1775-81 PMID: 4321345
  13. Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI.
    Proc Natl Acad Sci U S A. 1973 Jan;70(1):200-4 PMID: 4567334
  14. Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome.
    J Mol Biol. 1974 Jul 15;86(4):709-26 PMID: 4473553
  15. RNA synthesis specific for an integrated adenovirus genome during the cell cycle.
    Nature. 1974 Aug 2;250(465):416-8 PMID: 4854184
  16. Induced changes in the rates of uridine- 3 H uptake and incorporation during the G 1 and S periods of synchronized Chinese hamster cells.
    J Cell Biol. 1972 Mar;52(3):514-25 PMID: 5009516
  17. Transformation of primary rat embryo cells by adenovirus type 2.
    Proc Natl Acad Sci U S A. 1967 Sep;58(3):1205-12 PMID: 4861310
  18. The mechanism of viral carcinogenesis by DNA mammalian viruses. VI. A new class of virus-specific RNA molecules in cells transformed by group C human adenoviruses.
    Proc Natl Acad Sci U S A. 1969 Sep;64(1):255-62 PMID: 5263008
  19. Histone synthesis in vitro on HeLa cell microsomes. The nature of the coupling to deoxyribonucleic acid synthesis.
    J Biol Chem. 1969 Nov 10;244(21):5947-52 PMID: 5350949
  20. RNA synthesis in Chinese hamster cells. II. Increase in rate of RNA synthesis during G1.
    J Cell Biol. 1969 Jul;42(1):308-15 PMID: 5795885
  21. RNA synthesis in synchronously growing populations of HeLa S3 cells. II. Rate of synthesis of individual RNA fractions.
    J Cell Physiol. 1968 Feb;71(1):95-104 PMID: 5663118
  22. Indices of synchrony in cellular cultures.
    Biometrics. 1967 Dec;23(4):693-716 PMID: 6080206
  23. Inhibition of DNA synthesis in HeLa cells by hydroxyurea.
    Cancer Res. 1967 Jan;27(1):124-9 PMID: 6020353
  24. Inhibition of DNA synthesis by hydroxyurea: structure-activity relationships.
    Cancer Res. 1967 Mar;27(3):535-40 PMID: 6021509
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.15.4.806-811.1975
Published
1975-04-00
Pages
806-11
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354523
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.15.4.806-811.1975 PMC Full Text View journal details
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