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PMID: 11675489 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A human nuclease specific for G4 DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 22 ·2001-10-23 ·Pages 12444-9

Sun H, Yabuki A, Maizels N

Abstract

We have identified a human nuclease that specifically cleaves four-stranded DNA stabilized by G quartets (G4 DNA). This nuclease, GQN1 (G quartet nuclease 1), cuts within the single-stranded region 5' of the barrel formed by stacked G quartets. GQN1 does not cleave duplex or single-stranded DNA, Holliday junctions, or G4 RNA. Cleavage depends on DNA structure and not on flanking sequence. Activity is elevated in but not restricted to B cells, making GQN1 a strong candidate for function in immunoglobulin heavy chain class switch recombination. Identification of a mammalian nuclease that specifically cleaves G4 DNA provides further support for the notion that DNA structures stabilized by G quartets form in vivo and function in regulated recombination and genomic evolution.

MeSH Terms
Base Sequence Cell Line DNA/chemistry,metabolism Deoxyribonucleases/isolation & purification,metabolism Guanine Humans Molecular Sequence Data Substrate Specificity
Chemicals
Guanine DNA Deoxyribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sun H
Department of Molecular Biophysics and Biochemistry and Genetics, Yale University School of Medicine, New Haven, CT 06520, USA.
Yabuki A
Maizels N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.231479198
Published
2001-10-23
Pages
12444-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC60073
Subset
IM
Grants
NIGMS NIH HHS · R01 GM039799 · United States
PHS HHS · P01 16038 · United States
NIGMS NIH HHS · R01 GM39799 · United States
External Links
PubMed source DOI: 10.1073/pnas.231479198 PMC Full Text View journal details
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